Recombinant Mouse Alppl2 Protein, His&GST-tagged

• Cat.No.: Alppl2-126M
• Product Overview: Recombinant Mouse Alppl2 Protein (Val19-Ser502) with N-His&GST tag was expressed in E. coli.

Specification

• Description: Enables alkaline phosphatase activity. Predicted to be involved in dephosphorylation. Located in myofibril. Is expressed in early conceptus; intestine; and testis. Human ortholog(s) of this gene implicated in inflammatory bowel disease. Orthologous to human ALPG (alkaline phosphatase, germ cell); ALPI (alkaline phosphatase, intestinal); and ALPP (alkaline phosphatase, placental).
• Source: E. coli
• Species: Mouse
• Tag: N-His&GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 82.6 kDa
  Accurate Molecular Mass: 83 kDa
• Protein length: Val19-Ser502
• Purity: > 80%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Alppl2 alkaline phosphatase, placental-like 2 [ Mus musculus (house mouse) ]
• Official Symbol: Alppl2
• Synonyms: ALPPL2; alkaline phosphatase, placental-like 2; embryonic-type alkaline phosphatase; alkaline phosphatase 5; embryonic alkaline phosphatase; EAP; Akp5; C77216; D1Ertd816e
• Gene ID: 11650
• mRNA Refseq: NM_007433
• Protein Refseq: NP_031459
• UniProt ID: P24823

Reviews

03/16/2022

Versatile applications in multiple assay formats.

02/07/2022

Easy-to-use assay kit with clear protocols.

01/25/2017

Fast and efficient protein purification.

Q&As

How can ALPPL2 activity and function be manipulated to modulate cellular processes and disease outcomes?

Manipulating ALPPL2 activity and function holds promise for modulating cellular processes and disease outcomes, potentially offering new therapeutic avenues for metabolic disorders, liver diseases, and lipid-related conditions.

How does ALPPL2 dysregulation or deficiency contribute to the pathogenesis of diseases and disorders?

Dysregulation or deficiency of ALPPL2 has been associated with metabolic disorders, liver diseases, and dyslipidemia, highlighting its contribution to the pathogenesis of these conditions.

Can ALPPL2 serve as a diagnostic biomarker or therapeutic target for specific diseases?

ALPPL2 may serve as a potential diagnostic biomarker for specific diseases, providing insights into disease progression and therapeutic response, and it could be explored as a therapeutic target for developing interventions in certain pathological contexts.

What are the physiological functions and molecular interactions of ALPPL2 in cellular processes?

ALPPL2 participates in cellular processes such as lipid metabolism, cell signaling, and membrane dynamics, and it interacts with other proteins and molecules involved in these processes.

How does ALPPL2 expression and activity change in response to physiological and pathological stimuli?

ALPPL2 expression and activity are influenced by various physiological and pathological stimuli, including hormonal regulation, nutrient availability, and disease conditions.

What is the tissue-specific expression pattern of ALPPL2 and its cellular localization?

ALPPL2 exhibits a specific expression pattern, with higher levels in certain tissues such as the liver, kidney, and intestine, and it is primarily localized to the plasma membrane or specific subcellular compartments.

What are the downstream signaling pathways and molecular targets regulated by ALPPL2 in cellular processes?

ALPPL2 regulates downstream signaling pathways and molecular targets involved in lipid metabolism, cell proliferation, and cell survival, influencing cellular processes such as lipid signaling, membrane trafficking, and cell growth.

How does ALPPL2 contribute to the metabolism and turnover of its substrates in different cellular contexts?

ALPPL2 is involved in the metabolism and turnover of its substrates, including lipid molecules and membrane components, playing important roles in cellular lipid homeostasis and membrane remodeling.

What are the upstream regulators and signaling pathways that control ALPPL2 expression and function?

ALPPL2 expression is controlled by transcriptional regulators, signaling pathways, and epigenetic modifications that respond to specific cellular signals and environmental cues.

Can ALPPL2 be modulated by specific factors or inhibitors, and how does this affect its enzymatic activity and cellular functions?

ALPPL2 activity can be modulated by specific factors, such as hormones or lipid-binding proteins, and inhibitors that target its catalytic site or affect its membrane association, leading to altered enzymatic activity and cellular functions.