Recombinant Mouse Alx3 Protein, MYC/DDK-tagged

• Cat.No.: Alx3-589M
• Product Overview: Purified recombinant protein of full-length mouse aristaless-like homeobox 3 (Alx3), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: This gene belongs to Group 1 of aristaless-like genes, which are characterized by the presence of an aristaless domain and a conserved paired-like homeodomain. The encoded protein acts as a transcriptional regulator. The protein plays a role in the development of craniofacial and appendicular skeleton and may have a role in pancreatic function.
• Source: HEK293T
• Species: Mouse
• Tag: MYC/DDK
• Molecular Mass: 37.3 kDa
• Purity: >80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Alx3 aristaless-like homeobox 3 [ Mus musculus (house mouse) ]
• Official Symbol: Alx3
• Synonyms: Alx3; aristaless-like homeobox 3; homeobox protein aristaless-like 3; proline-rich transcription factor Alx3
• Gene ID: 11694
• mRNA Refseq: NM_007441
• Protein Refseq: NP_031467
• UniProt ID: O70137

Reviews

04/20/2020

Accurate measurement of protein-protein complex stoichiometry.

12/31/2019

Compatible with high-content imaging platforms.

11/16/2017

Reduced assay variability for reliable data interpretation.

Q&As

How is ALX3 expression regulated during embryonic development, particularly in tissues where it plays a critical role?

ALX3 expression is regulated spatially and temporally during embryonic development, with specific patterns in tissues such as the neural tube, limb buds, or craniofacial structures.

Can ALX3 be targeted pharmacologically or genetically to modulate its activity, and what are the potential therapeutic implications?

Pharmacological or genetic modulation of ALX3 activity may have therapeutic implications for developmental disorders or regenerative medicine, but further studies are needed to explore these possibilities.

What are the DNA binding motifs recognized by ALX3, and how do they contribute to its transcriptional regulatory activity?

ALX3 recognizes specific DNA binding motifs, such as homeobox motifs, and binds to regulatory regions of target genes to regulate their transcriptional activity.

How does ALX3 interact with other transcription factors and co-regulators to form transcriptional complexes that control gene expression and cellular fate during embryonic development?

ALX3 forms transcriptional complexes with other transcription factors and co-regulators, such as PAX6 or DLX5, to coordinate gene expression programs essential for embryonic development and tissue morphogenesis.

What are the epigenetic modifications associated with ALX3 binding sites and how do they modulate ALX3-mediated transcriptional regulation?

ALX3 binding sites are associated with specific epigenetic modifications, such as DNA methylation or histone modifications, which contribute to the regulation of ALX3-mediated transcriptional activity.

What are the protein-protein interactions of ALX3 and how do these interactions contribute to its function?

ALX3 interacts with co-regulatory proteins and other transcription factors, forming complexes that modulate gene expression and cellular processes critical for embryonic development.

What are the downstream targets of ALX3, and how does it regulate gene expression to influence cellular processes during embryogenesis?

ALX3 regulates the expression of genes involved in cell fate determination, tissue patterning, and morphogenesis during embryogenesis, including those encoding transcription factors, extracellular matrix components, or signaling molecules.

How does ALX3 interact with signaling pathways involved in tissue development, such as Wnt, BMP, or FGF signaling?

ALX3 integrates with signaling pathways, including Wnt, BMP, or FGF signaling, to modulate gene expression and influence cellular behaviors during embryogenesis.

How does ALX3 regulate cell proliferation, differentiation, and migration during embryogenesis, and what are the underlying molecular mechanisms?

ALX3 regulates cell proliferation, differentiation, and migration during embryogenesis through various molecular mechanisms, including interactions with cell cycle regulators, morphogen gradients, or cytoskeletal components.

What are the effects of ALX3 gain-of-function or loss-of-function mutations on embryonic development, and how do they affect cellular behaviors and tissue morphogenesis?

Gain-of-function or loss-of-function mutations in ALX3 can disrupt normal embryonic development, leading to craniofacial malformations, limb abnormalities, or neural tube defects.