Recombinant Mouse ARHGEF2 Protein, C-Flag-tagged
Cat.No. : | ARHGEF2-05M |
Product Overview : | Recombinant Mouse ARHGEF2 Protein, fused to Flag-tag at C-terminus, was expressed in HEK293. |
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Description : | Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, innate immune response, and cancer. Binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases. May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases. Forms an intracellular sensing system along with NOD1 for the detection of microbial effectors during cell invasion by pathogens. Involved in innate immune signaling transduction pathway promoting cytokine IL6/interleukin-6 and TNF-alpha secretion in macrophage upon stimulation by bacterial peptidoglycans; acts as a signaling intermediate between NOD2 receptor and RIPK2 kinase. Contributes to the tyrosine phosphorylation of RIPK2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Overexpression activates Rho-, but not Rac-GTPases, and increases paracellular permeability (By similarity). Involved in neuronal progenitor cell division and differentiation. Involved in the migration of precerebellar neurons. |
Source : | HEK293 |
Species : | Mouse |
Tag : | C-Flag |
Form : | 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol. |
Molecular Mass : | 111.9 kDa |
AA Sequence : | MSRIESLTRARIDRSKEQATKTREK EKMKEAKDARYTNGHLFTTISVSGM TMCYACNKSITAKEALICPT CNVTIHNRCKDTLANCTKVKQKQQK AALLRNNTALQSVSLRSKTTTRERP TSAIYPSDSFRQSLLGSRRG LSSLSLAKSVSTTNIAGHFNDESPL GLRQILSQSTDSLNMRNRTLSVESL IDEGVEVFYNELMSDFEMDE KDFEADSWSLAVDSSFLQQHKKEVM KKQDVIYELIQTELHHVRTLKIMTR LFRTGMLEELQMEPEVVQGL FPCVDELSDIHTRFLNQLLERRRQA LCPGSTRNFVIHRLGDLLISQFSGS NAEQMRKTYSEFCSRHTKAL KLYKELYARDKRFQQFIRKMTRSAV LKRHGVQECILLVTQRITKYPVLIN RILQNSHGVEEEYQDLASAL GLVKELLSNVDQDVHELEKEARLQE IYNRMDPRAQTPVPGKGPFGRDELL RRKLIHEGCLLWKTATGRFK DVLLLLMTDVLVFLQEKDQKYIFTS LDKPSVVSLQNLIVRDIANQAKGMF LISSGPPEMYEVHAASRDDR TTWIRVIQQSVRLCPSREDFPLIET EDKAYLRRIKTKLQQKNQALVELLQ KNVELFAEMVHFQALKAGFV GMPPPALPRGLFRLESFESLRGERL LKDALREVEGLKDLLLGPCVDLPTT SREPALPLDSDSGSCPGVTA NGEARTFNGSIELCRADSDSSQKDR NGNQLRSPQEEVLQPLINLYGLLHG LQAVVVQQERLMEALFPEGP ERWEKLSRANSRDGEAGRAAVASVT PEKQATELALLQRQHTLLQEELRRC QRLGEERATEAGSLEARLRE SEQARALLEREAEEIRRQLAALGQN EPLPAEAPWARRPLDPRRRSLPAGD ALYLSFNPPQPSRGHDRLDL PVTVRSLHRPFDDREAQELGSPEDR LQDSSDPDTGSEEEVSSRLSPPHSP RDFTRMQDIPEETESRDGEP TASES TRTRPLEQKLISEEDLAANDILDYK DDDDKV |
Purity : | > 80% as determined by SDS-PAGE and Coomassie blue staining. |
Notes : | For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process. |
Storage : | Store at -80 centigrade after receiving vials. |
Concentration : | >0.05 μg/μL as determined by microplate BCA method. |
Use/Stability : | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Gene Name : | Arhgef2 Rho/Rac guanine nucleotide exchange factor 2 [ Mus musculus (house mouse) ] |
Official Symbol : | ARHGEF2 |
Synonyms : | GEF; Lfc; P40; GEFH1; LFP40; Lbcl1; GEF-H1; mKIAA0651; AA408978; Rho/Rac guanine nucleotide exchange factor 2; ARHGEF2 |
Gene ID : | 16800 |
mRNA Refseq : | NM_008487.4 |
Protein Refseq : | NP_032513.3 |
UniProt ID : | Q60875 |
Products Types
◆ Recombinant Protein | ||
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ARHGEF2-426R | Recombinant Rat ARHGEF2 Protein, His (Fc)-Avi-tagged | +Inquiry |
ARHGEF2-770R | Recombinant Rat ARHGEF2 Protein | +Inquiry |
ARHGEF2-785H | Recombinant Human ARHGEF2 protein, GST-tagged | +Inquiry |
◆ Lysates | ||
ARHGEF2-8732HCL | Recombinant Human ARHGEF2 293 Cell Lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (8)
Ask a questionTargeting ARHGEF2 or its downstream effectors could hold therapeutic potential for various diseases. Given its role in cancer metastasis, inhibiting ARHGEF2 could help prevent or reduce tumor cell invasion and migration. Modulating ARHGEF2 activity could also be beneficial in cardiovascular diseases by regulating blood vessel contraction and cardiac hypertrophy. Furthermore, targeting ARHGEF2 in neurological disorders might help regulate neuronal development and synaptic plasticity.
ARHGEF2 can interact with multiple proteins to exert its functions. It has been reported to interact with and activate several Rho GTPases, including RhoA, RhoB, and RhoC. ARHGEF2 can also interact with other signaling molecules, such as focal adhesion kinase (FAK), paxillin, and Galpha12/13 proteins, to regulate cytoskeletal dynamics and cell adhesion.
Yes, ARHGEF2 can interact with several other proteins involved in cell signaling pathways. It can interact with integrin-associated proteins like vinculin and talin, as well as adherens junction proteins such as E-cadherin, β-catenin, and α-catenin. These interactions modulate cell adhesion and cytoskeletal dynamics. Additionally, ARHGEF2 can interact with signaling molecules like G-protein-coupled receptors (GPCRs), growth factor receptors, and non-receptor tyrosine kinases, enabling crosstalk between different signaling pathways.
ARHGEF2 has been shown to activate RhoA, which in turn regulates a variety of downstream effectors and signaling pathways. This includes the Rho-associated protein kinase (ROCK) pathway, which controls actin-myosin contractility and cell shape changes, as well as the mDia and IRSp53 pathways involved in actin filament assembly. Additionally, ARHGEF2 can activate Rac1, which can activate JNK and p38 MAP kinase pathways to influence cell migration and invasion.
Yes, mutations in the ARHGEF2 gene have been associated with different disorders. In particular, loss-of-function mutations in ARHGEF2 have been linked to intellectual disability and developmental delay. These mutations can lead to altered Rho GTPase signaling, affecting cellular processes involved in neural development and cognition. Additionally, variations in the ARHGEF2 gene have been found to be associated with hypertension susceptibility in certain populations.
Yes, research on ARHGEF2 is ongoing. Scientists continue to investigate its role in various cellular processes and its involvement in different diseases. They are studying the mechanisms of ARHGEF2 activation, its interaction partners, and the downstream pathways it regulates. Additionally, efforts are being made to explore potential therapeutic strategies targeting ARHGEF2 for diseases such as cancer and neurological disorders.
ARHGEF2 is involved in multiple cellular processes, including actin cytoskeleton organization, cell adhesion, cell migration, cell proliferation, and cell polarity. It regulates these processes by activating Rho GTPases, which in turn control the organization of the actin cytoskeleton and the dynamics of cell shape changes. ARHGEF2 is essential for various physiological processes, such as development, tissue homeostasis, and wound healing, and its dysregulation is associated with pathological conditions like cancer and cardiovascular diseases.
Yes, ARHGEF2 has been implicated in various diseases and pathological conditions. It has been found to be involved in cancer progression and metastasis by promoting cell migration, invasion, and epithelial-mesenchymal transition (EMT). ARHGEF2 has also been linked to cardiovascular diseases, such as hypertension and cardiac hypertrophy, as well as neuronal disorders, including epilepsy and intellectual disability.
Customer Reviews (8)
Write a reviewthe manufacturer's commitment to delivering the ARHGEF2 protein in a timely manner is greatly appreciated.
the ARHGEF2 protein demonstrates significant relevance and potential in various trial applications, primarily focusing on inflammation, angiogenesis, and tissue remodeling.
Their prompt and knowledgeable assistance has the potential to solve any difficulties I may encounter during my experiments.
The manufacturer maintains stringent quality control measures to ensure the purity and stability of the protein, minimizing experimental variability and maximizing the accuracy of the data obtained.
The cost-effectiveness of the ARHGEF2 protein, competitive pricing, and the availability of bulk purchase options provided by the manufacturer also align with my budget considerations, allowing me to optimize my resources.
Another advantage of the ARHGEF2 protein is its reliability and consistency, ensuring robust and reproducible results.
the manufacturer's support plays a crucial role in the successful implementation of trials involving the ARHGEF2 protein.
I am confident that my experimental needs will be met through their assistance, ensuring successful outcomes in my trials.
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