Recombinant Mouse Asah1 Protein, MYC/DDK-tagged

• Cat.No.: Asah1-655M
• Product Overview: Purified recombinant protein of full-length mouse N-acylsphingosine amidohydrolase 1 (Asah1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: This gene encodes acid ceramidase, an enzyme that plays a central role in ceramide metabolism. The encoded protein undergoes proteolytic processing to generate a heterodimeric enzyme comprised of alpha and beta subunits that catalyzes the hydrolysis of sphingolipid ceramide into sphingosine and free fatty acid. The homozygous disruption of this gene leads to embryonic lethality in mice whereas the heterozygous animals exhibit a progressive lipid storage disease phenotype.
• Source: HEK293T
• Species: Mouse
• Tag: MYC/DDK
• Molecular Mass: 44.7 kDa
• Purity: >80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Asah1 N-acylsphingosine amidohydrolase 1 [ Mus musculus (house mouse) ]
• Official Symbol: Asah1
• Synonyms: Asah1; N-acylsphingosine amidohydrolase 1; AC; Asah; 2310081N20Rik; acid ceramidase; ACDase; N-acylethanolamine hydrolase ASAH1; acid CDase; acylsphingosine deacylase; EC 3.5.1.23
• Gene ID: 11886
• mRNA Refseq: NM_019734
• Protein Refseq: NP_062708
• UniProt ID: Q9WV54

Reviews

03/21/2022

Such dedicated support makes me feel confident in my choice of using the ASAH1 protein for my experiments.

04/09/2017

The manufacturer's commitment to excellence is truly commendable, especially when it comes to their outstanding technical support.

11/02/2016

The ASAH1 Protein is a protein of exceptional quality that surpasses my expectations and fulfills all my experimental requirements.

Q&As

Can ASAH1 activity be targeted for therapeutic interventions?

Given the involvement of ASAH1 in various diseases, including cancer, modulating its activity or developing ASAH1 inhibitors holds promise as potential therapeutic strategies.

Can alterations in ASAH1 gene expression affect cellular differentiation?

Yes, alterations in ASAH1 gene expression or activity can impact cellular differentiation processes, as ceramide metabolism is crucial for normal development and tissue homeostasis.

Are there any diseases or conditions associated with the ASAH1 protein?

Yes, mutations or deficiencies in the ASAH1 gene are associated with Farber disease, a rare inherited disorder characterized by the accumulation of ceramide in tissues throughout the body.

Can ASAH1 deficiency be prevented?

ASAH1 deficiency, as seen in Farber disease, is a genetic disorder. Prevention involves genetic counseling for at-risk individuals and prenatal testing during pregnancy.

Can ASAH1 deficiency be detected in newborn screening tests?

Currently, ASAH1 deficiency is not included in routine newborn screening panels. However, advancements in testing methods may lead to its inclusion in the future.

Does the ASAH1 protein have any other biological functions?

Besides its role in ceramide metabolism, the ASAH1 protein can affect immune response, inflammation, and cellular stress responses.

How does dysfunction of the ASAH1 protein impact ceramide metabolism?

Dysfunction of the ASAH1 protein impairs the breakdown of ceramide, leading to its accumulation in various tissues and disrupting cellular homeostasis.

Is the ASAH1 protein involved in any signaling pathways?

Yes, the ASAH1 protein can modulate signaling pathways like the sphingosine-1-phosphate pathway, which influences various cellular processes such as migration, inflammation, and angiogenesis.