Recombinant Mouse Atp2b2 Protein, His/GST/S-tagged

• Cat.No.: Atp2b2-464M
• Product Overview: Recombinant Mouse Atp2b2(Ala417~Ile830) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Source: E. coli
• Species: Mouse
• Tag: His, GST-tag, S-tag
• Form: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Molecular Mass: 75.8kDa
• Protein length: Ala417~Ile830
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: > 85%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Atp2b2 ATPase, Ca++ transporting, plasma membrane 2 [ Mus musculus ]
• Official Symbol: Atp2b2
• Synonyms: ATP2B2; ATPase, Ca++ transporting, plasma membrane 2; plasma membrane calcium-transporting ATPase 2; wriggle; deaf waddler; plasma membrane calcium pump; plasma membrane calcium ATPase; Tmy; dfw; jog; wms; wri; PMCA2; D6Abb2e
• Gene ID: 11941
• mRNA Refseq: NM_001036684
• Protein Refseq: NP_001031761

Reviews

04/27/2022

Due to the high catalytic efficiency, high safety and high expression level of ATP2B2, the experimental process is more stable and reliable.

05/24/2021

The stability of the ATP2B2 is very good and can be stored for a long time without inactivation.

06/05/2020

ATP2B2 performed very well in Western blot with clear bands, which improved the accuracy of the experiment.

Q&As

What are the main functions of ATP2B2?

ATP2B2 is a calcium transporter, which is mainly responsible for transporting intracellular calcium to the outside of the cell, maintaining the balance of intracellular and extracellular calcium concentration, thus regulating the function of cells.

In which diseases does ATP2B2 have potential applications?

At present, the application potential of ATP2B2 is still under investigation, but it has been suggested that this protein may be related to the treatment of hypertension, cardiovascular disease, nervous system disease and other diseases.

What research studies and ATP2B2 have been carried out so far?

Previous studies have focused on the mechanism and regulation of ATP2B2 in nervous system diseases, cardiovascular diseases and other related diseases, and the relationship between ATP2B2 and diseases.

How does ATP2B2 work?

ATP2B2 may affect the excitability of cells, muscle contraction, nerve conduction and other important physiological processes by regulating the dynamic balance of calcium ion in and out of cells.

Are there other related proteins that function similarly to ATP2B2?

In addition to ATP2B2, there are other proteins involved in calcium ion transport, such as ATP2B1 and ATP2B3, which also play a role in regulating calcium ion balance.

How is ATP2B2 prepared?

It is usually prepared by transferring the atpase B2 gene into the host cells suitable for protein expression through genetic engineering technology, and then obtaining the recombinant protein through cell culture and protein purification technology.