Recombinant Mouse Cldn3 protein, His-tagged

• Cat.No.: Cldn3-1427M
• Product Overview: Recombinant Mouse Cldn3 aa. (Arg30~Met159 (Accession # Q9Z0G9) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. The protein encoded by this gene is a low-affinity receptor for clostridium perfringens enterotoxin (CPE) produced by the bacterium Clostridium perfringens, and the interaction with CPE results in increased membrane permeability by forming small pores in plasma membrane. This protein is highly overexpressed in uterine carcinosarcoma. This protein is also predominantly present in brain endothelial cells, where it plays a specific role in the establishment and maintenance of blood brain barrier tight junction morphology.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 16kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Arg30~Met159 (Accession # Q9Z0G9)
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.6.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as liquid form in Phosphate buffered saline(PBS), pH 7.4.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Cldn3 claudin 3 [ Mus musculus (house mouse) ]
• Official Symbol: Cldn3
• Synonyms: Cldn3; claudin 3; mRVP1; Cpetr2; AI182374; claudin-3; CPE-R 2; CPE-receptor 2; clostridium perfringens enterotoxin receptor 2
• Gene ID: 12739
• mRNA Refseq: NM_009902.4
• Protein Refseq: NP_034032.1
• UniProt ID: Q9Z0G9

Reviews

04/24/2019

The manufacturer's deep understanding of the protein and its applications enables them to promptly and effectively address any issues that may arise during my trials.

06/30/2018

It minimizes the risk of protein degradation and maximizes the effectiveness of the protein in various applications, such as cell signaling pathways or metabolic studies.

12/12/2017

This stability is particularly advantageous for long-term studies or experiments involving extended incubation periods.

Q&As

Is CLDN3 a potential target for drug delivery to specific tissues?

Researchers are exploring the use of CLDN3 as a target for drug delivery systems, aiming to enhance the specificity of drug delivery to tissues expressing high levels of CLDN3.

How does CLDN3 impact the permeability of the intestinal epithelium?

CLDN3 is involved in the tight junctions of intestinal epithelial cells, influencing the permeability of the intestinal barrier and playing a role in conditions like IBD.

In which other diseases or conditions is CLDN3 being investigated?

CLDN3 is under investigation in various conditions, including infectious diseases, where its role in tight junctions may have implications for host-pathogen interactions.

Can CLDN3 be involved in inflammatory bowel diseases (IBD)?

Yes, CLDN3 is implicated in the pathogenesis of inflammatory bowel diseases, and its expression levels are being studied as potential markers for disease severity.

How is CLDN3 linked to neurodegenerative diseases?

Altered expression of CLDN3 in the blood-brain barrier is associated with certain neurodegenerative diseases, making it a subject of interest for researchers studying these conditions.