Active Recombinant Mouse F7 protein(Met1-Leu446), His-tagged

• Cat.No.: F7-2206M
• Product Overview: Recombinant Mouse FⅦ (NP_034302.2) (Met 1-Leu 446) was expressed in CHO, fused with the a polyhistidine tag at the C-terminus.

Specification

• Source: CHO
• Species: Mouse
• Tag: C-His
• Protein length: Met1-Leu446
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized mouse F7-his at 10 μg/ml (100 μl/well) can bind biotinylated mouse F3-his. The EC50 of biotinylated mouse F3-his is 0.1-0.3 μg/ml.
• Molecular Mass: The mature form of mouse FⅦ consists of 416 amino acids after removal of the signal peptide and the propeptid, and has a predicted molecular mass of 47 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FⅦ is approximately 56-63 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: F7 coagulation factor VII [ Mus musculus ]
• Official Symbol: F7
• Synonyms: F7; coagulation factor VII; serum prothrombin conversion accelerator; Cf7; FVII; mfVII; AI132620
• Gene ID: 14068
• mRNA Refseq: NM_010172
• Protein Refseq: NP_034302

Reviews

05/18/2022

Quick turnaround—this product arrived swiftly, facilitating our research.

10/03/2019

Exceptional quality and precision—our lab relies on this protein consistently.

05/29/2018

Invaluable assistance from customer service—professional and responsive.

Q&As

How does F7 contribute to hemostasis, and what is its role in preventing excessive bleeding?

Factor VII's role in the coagulation cascade ensures rapid initiation of clot formation, preventing excessive bleeding and contributing to hemostasis.

Discuss the role of F7 in the extrinsic pathway of blood coagulation.

Factor VII is a key player in the extrinsic pathway, where it forms a complex with tissue factor to activate factor X, initiating the cascade leading to thrombin generation.

What are the mechanisms of F7 activation, and how is it regulated at the molecular level?

Factor VII is activated through a proteolytic cleavage, converting it into its active form (VIIa). This activation is regulated by tissue factor and other coagulation factors.

Explain the biochemical basis of F7 deficiency and its implications for bleeding disorders.

F7 deficiency results in impaired blood clotting, leading to a bleeding disorder known as coagulation factor VII deficiency. This condition can manifest in varying degrees of severity.

Explain the role of F7 in the coagulation cascade and blood clot formation.

Factor VII is activated to its enzymatically active form (factor VIIa) and initiates the coagulation cascade by activating factor X, ultimately leading to the formation of a stable blood clot.