Recombinant Mouse Ifng protein(Met1-Cys155), hFc-tagged

• Cat.No.: Ifng-119M
• Product Overview: Recombinant Mouse IFNG (P01580) (Met 1-Cys 155) was expressed in HEK293, fused with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-hFc
• Protein length: Met1-Cys155
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, 0.02% TWeen80, pH8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured in antiviral assays using L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is 2.5-15 ng/mL. 2. Measured by its ability to bind with recombinant mouse IFNGR1-His in a functional ELISA.
• Molecular Mass: The secreted recombinant mouse IFNG/Fc is a disulfide-linked homodimer. The reduced monomer comprises 374 amino acids and has a calculated molecular mass of 42.5 kDa. As a result of glycosylation, the apparent molecular mass of the monomer is approximately 55 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Ifng interferon gamma [ Mus musculus ]
• Official Symbol: Ifng
• Synonyms: IFNG; interferon gamma; IFN-gamma; gamma interferon; Ifg; IFN-g
• Gene ID: 15978
• mRNA Refseq: NM_008337
• Protein Refseq: NP_032363

Reviews

12/17/2022

It is analyzed by X-ray crystallography or other crystallization techniques and has good crystallization properties.

06/10/2022

Easy to attach to the surface of cells or materials.

04/02/2022

IFNG products have a complete range of specifications for experimental designs of different sizes and needs.

Q&As

What is the role of IFNG in tumor immunotherapy?

IFNG plays an important role in tumor immunotherapy, which can activate the immune response of tumor cells, promote apoptosis of tumor cells and inhibit the proliferation of tumor cells.

What is the current progress in drug development for IFNG?

The development of drugs for IFNG is currently in clinical trials, including monoclonal antibodies against IFNG receptors and inhibitors against IFNG signaling pathways.

How is IFNG related to muscle wasting?

Studies have shown that IFNG can inhibit the occurrence of muscle atrophy, but the specific mechanism is not clear.

How can IFNG be used as a biomarker in clinical practice?

In clinical practice, the expression level of IFNG can be measured to predict the progression and prognosis of the disease, and to guide the choice of treatment regimen.

Are there other coding variants in the IFNG gene?

Yes, there are other coding variants in the IFNG gene that may have different functions and expression patterns.

Is the expression of IFNG affected by environmental factors?

Such as nutritional status, inflammation levels, etc. Yes, the expression of IFNG is affected by environmental factors, such as nutritional status, inflammation level, etc.