Recombinant Mouse Il12a Protein (Met23-Ser335, Arg23-Ala215)

• Cat.No.: Il12a-6737M
• Product Overview: Recombinant mouse Il12a (Met23-Ser335&Arg23-Ala215) without tag was expressed in mammalian cells.

Specification

• Description: Enables interleukin-12 beta subunit binding activity. Contributes to cytokine activity. Acts upstream of or within several processes, including T-helper 1 cell activation; defense response to protozoan; and positive regulation of T cell activation. Located in cell surface; cytoplasm; and extracellular space. Part of interleukin-12 complex. Is expressed in brain; lung; small intestine; testis; and thymus. Human ortholog (s) of this gene implicated in hepatitis C; hepatocellular carcinoma; and primary biliary cholangitis. Orthologous to human IL12A (interleukin 12A).
• Source: Mammalian cells
• Species: Mouse
• Tag: Tag Free
• Form: Lyophilized powder/frozen liquid
• Molecular Mass: 55.66 kDa
• Protein Length: Met23-Ser335, Arg23-Ala215
• Purity: > 90 % as determined by SDS-PAGE
• Notes: For research use only.
• Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8 centigrade for one week. Store at -20 to -80 centigrade for twelve months from the date of receipt.
• Storage Buffer: Supplied as solution form in PBS or lyophilized from PBS.
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Shipping: They are shipped out with dry ice/blue ice unless customers require otherwise.

Gene Information

• Gene Name: Il12a interleukin 12a [ Mus musculus (house mouse) ]
• Official Symbol: Il12a
• Synonyms: Il12a; interleukin 12a; p35; Ll12a; Il-12a; IL-12p35; interleukin-12 subunit alpha; CLMF p35; IL-12 p35 subunit; IL-12 subunit p35; cytotoxic lymphocyte maturation factor 35 kDa subunit; interleukin 12a p35 subunit; interleukin-12 p35 subunit
• Gene ID: 16159
• mRNA Refseq: NM_001159424
• Protein Refseq: NP_001152896
• UniProt ID: P43431

Reviews

01/14/2021

Suitable for co-immunoprecipitation.

12/21/2020

Consistent in bioactivity.

11/16/2020

Good for receptor binding studies.

Q&As

How do mass spectrometry-based techniques reveal post-translational modifications on IL12A?

Mass spectrometry identifies IL12A's post-translational modifications and their roles.

What strategies validate IL12A's role in immune response modulation using knockout models?

Knockout models validate IL12A's roles in immune response modulation.

How do protein footprinting assays unveil IL12A's binding interfaces with receptor partners?

Protein footprinting assays reveal IL12A's binding interfaces with receptors.

What experimental methods quantify IL12A's secretion kinetics and stability in cell culture?

ELISA and stability assays quantify IL12A secretion and stability in culture.

Can you discuss the use of SPR in characterizing IL12A's interactions with antibodies and inhibitors?

SPR characterizes IL12A's interactions with antibodies and inhibitors.

What cellular assays assess IL12A's ability to induce cytokine production in immune cells?

Cellular assays gauge IL12A's cytokine induction in immune cells.

How does isothermal titration calorimetry provide insights into IL12A's binding affinity with receptors?

Isothermal titration calorimetry measures IL12A's binding affinity with receptors.

What techniques evaluate the impact of IL12A genetic variants on protein structure and function?

Structural biology techniques analyze IL12A genetic variant impacts.

Could you elaborate on the use of site-directed mutagenesis in probing IL12A's functional domains?

Site-directed mutagenesis probes IL12A's functional domains and interactions.

How does in vivo bioluminescence imaging track IL12A expression dynamics in living organisms?

Bioluminescence imaging tracks IL12A expression dynamics in vivo.