Recombinant Mouse IL23 protein, His-tagged

• Cat.No.: IL23-3392M
• Product Overview: Recombinant Mouse IL12B (P43432)(Met1-Ser335) was expressed, constructed the plasmid 1; Recombinant Mouse IL23A (Q9EQ14) (Met1-Ala196) was expressed with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the IL12B & IL23A heterodimer was purified in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His
• Protein length: Met1-Ser335&Met1-Ala196
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized Recombinant Human IL23R / IL23 Receptor Protein (ECD, Fc Tag) at 2 μg/mL (100 μL/well) can bind Recombinant Mouse IL-23 Heterodimer Protein (His Tag), the EC50 is 11-33 ng/mL.
• Molecular Mass: The recombinant heterodimer of Mouse IL12B & IL23A comprises (313+186) amino acids and has a calculated molecular mass of (35.8+21.11) KDa. The apparent molecular mass of Mouse IL12B & IL23A heterodimer is approximately 43.9、41.4、26.9 and 23.6 KDa respectively in SDS-PAGE. The molecular weight of this protein is around 62.2 kDa verified by SEC-MALS (Routinely tested).
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: ≥ 95 % as determined by SDS-PAGE, ≥ 95 % as determined by SEC-HPLC, ≥ 95% as determined by SEC-MALS(Routinely tested).
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Reviews

12/17/2021

High purity levels in CHIP

12/26/2018

Effective in T-cell differentiation.

09/25/2018

Suitable for signaling studies.

Q&As

Does IL23 expression correlate with disease severity in psoriasis?

IL23 expression correlates with disease severity in psoriasis.

Does IL23 expression vary between different immune cell types?

IL23 expression varies among dendritic cells and macrophages.

What are the downstream targets of IL23 signaling?

IL23 activates STAT3 to modulate downstream targets.

Does IL23 interact with other cytokines in a signaling cascade?

IL23 interacts with IL-12 to form a signaling cascade.

Is IL23 involved in the activation of STAT3?

IL23 is involved in the activation of STAT3.

How does IL23 expression change in autoimmune diseases?

IL23 expression is upregulated in autoimmune diseases like Crohn's.

What is the half-life of IL23 in plasma?

The half-life of IL23 in plasma is approximately 2 hours.

How does IL23 affect macrophage polarization?

IL23 induces M1 macrophage polarization.

What is the role of IL23 in Th17 cell differentiation?

IL23 promotes Th17 cell differentiation by activating STAT3.

How does IL23 contribute to tissue inflammation?

IL23 contributes to tissue inflammation by activating macrophages.