Recombinant Mouse Il6st protein(Met1-Glu617), His-tagged

• Cat.No.: Il6st-4011M
• Product Overview: Recombinant Mouse gp130 (NP_034690.3) (Met 1-Glu 617) was expressed in HEK293, fused with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Mouse
• Tag: C-His
• Protein length: Met1-Glu617
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its ability to bind mouse IL11Ra in a functional ELISA. 2. Measured by its ability to inhibit the IL-6Rα enhancement of IL-6 activity on M1 mouse myeloid leukemia cells. The ED50 for this effect is typically 0.6-3 μg/mL in the presence of 50 ng/mL recombinant human IL-6sR and 100 ng/mL recombinant human IL-6.
• Molecular Mass: The secreted recombinant mouse gp130 consists of 606 amino acids and has a predicted molecular mass of 68 kDa. As a result of glycosylation, the apparent molecular mass of rm gp130 is approximately 80-90 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Il6st interleukin 6 signal transducer [ Mus musculus ]
• Official Symbol: Il6st
• Synonyms: IL6ST; interleukin 6 signal transducer; interleukin-6 receptor subunit beta; IL-6RB; IL-6R-beta; IL-6R subunit beta; membrane glycoprotein 130; IL-6 receptor subunit beta; interleukin-6 signal transducer; oncostatin-M receptor subunit alpha; CD130; gp130; AA389424; BB405851; D13Ertd699e; 5133400A03Rik
• Gene ID: 16195
• mRNA Refseq: NM_010560
• Protein Refseq: NP_034690

Reviews

02/28/2022

IL6ST proteins can be functionally enhanced or altered by protein modification techniques.

08/04/2020

As an immunotherapy drug, it has good effect and high safety.

09/06/2019

Has a good inhibitory effect on cell proliferation and survival.

Q&As

How to detect IL6ST activity?

The activity of IL6ST is usually detected by cell differentiation and proliferation assay.

In what diseases does IL6ST have applications?

IL6ST may be used in the future to treat pulmonary fibrosis, chronic pulmonary obstruction, rheumatoid arthritis, and other chronic kidney diseases.

Are there any effects of IL6ST on cell differentiation?

IL6ST is involved in various inflammatory responses and cell differentiation processes.

What are the physical and chemical properties of IL6ST?

The physical and chemical properties of IL6ST include molecular weight, voltammetry potential, structure and stability.

Why research IL6ST?

The study of IL6ST helps us to better understand the disease mechanism involved in IL6ST, and provides new ideas and means for disease treatment.

What is the preparation method of IL6ST?

The IL6ST is usually prepared by transforming E. coli into an expression vector and making it express the IL6ST protein.