Recombinant Mouse Parp1 Protein, His-tagged, Alexa Fluor 555 conjugated

• Cat.No.: Parp1-2535MAF555
• Product Overview: Alexa Fluor 555 conjugated recombinant Mouse Parp1 (NP_031441.2) (Met 1-Trp 1014), fused with a polyhistidine tag at the N-terminus, was produced in Baculovirus-Insect cells.

Specification

• Source: Insect Cells
• Species: Mouse
• Tag: His
• Form: Lyophilized
• Molecular Mass: The recombinant mouse PARP1 consists of 1033 amino acids and has a calculated molecular mass of 115 kDa. It migrates as an approximately 75 kDa band in SDS-PAGE under reducing conditions.
• Protein length: 1033
• Endotoxin: < 1.0 EU/ μg of the protein as determined by the LAL method.
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 555 via amines
  With an excitation and emission maximum of 555/565 nm, Alexa Fluor 555 can be efficiently excited using a 543 nm He-Ne laser line and detected under standard TRITC/Cy3 filters.
• Stability: Samples are stable for up to 12 months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 to -70 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Lyophilized from 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 10% gly, 0.1 mM TCEP
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Parp1 poly (ADP-ribose) polymerase family, member 1 [ Mus musculus ]
• Official Symbol: Parp1
• Gene ID: 11545
• mRNA Refseq: NM_007415
• Protein Refseq: NP_031441

Reviews

06/01/2020

This range of applications of PARP1 is breathtaking, and it can play an important role in any field.

12/02/2019

This short half-life and high clearance of PARP1 make it have greater potential in the treatment of tumors, infections and other diseases, and it is expected to become a new generation of therapeutic drugs.

03/18/2019

The purity and bioactivity of PARP1 make it a valuable resource for scientific research.

Q&As

Does PARP1 protein cause cells to die in time?

Yes, overactivation of the PARP1 protein can lead to apoptosis or necrosis of cells, which may be exploited in the treatment of certain diseases.

How is the PARP1 protein involved in DNA repair?

The PARP1 protein can repair DNA damage by attaching ADP-ribose groups to damaged DNA strands and recruiting other repair proteins.

What are the other regulatory mechanisms of PARP1 protein in DNA damage repair?

In addition to PARP1's involvement in direct DNA repair, it can also modulate the selection of repair pathways and initiate other DNA damage sensing mechanisms.

What is the potential of PARP1 protein in tumor immunotherapy?

Some studies have proposed that the interaction of PARP1 protein with DNA damage in tumor immunotherapy may affect the efficacy of immunotherapy.

Is PARP1 protein involved in cell cycle regulation?

Yes, the PARP1 protein is involved in the normal regulation of the cell cycle, especially G1/S and G2/M switching during DNA damage.

What are the effects of overactivation of PARP1 protein on cells?

Overactivation of PARP1 depletes energy stores such as NAD+ and ATP and leads to apoptosis, or cell death.