Recombinant Mouse Pdx1 protein, His & GST-tagged

• Cat.No.: Pdx1-1573M
• Product Overview: Recombinant Mouse Pdx1 aa. (Met1~Arg284 (Accession # P52946)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Source: E. coli
• Species: Mouse
• Tag: His & GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 61.0kDa
• Protein length: Met1~Arg284 (Accession # P52946)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >90%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.
• Isoelectric Point: 6.4

Gene Information

• Gene Name: Pdx1 pancreatic and duodenal homeobox 1 [ Mus musculus (house mouse) ]
• Official Symbol: Pdx1
• Synonyms: Pdx1; pancreatic and duodenal homeobox 1; Ipf1; IDX-1; IPF-1; Mody4; STF-1; pdx-1; pancreas/duodenum homeobox protein 1; insulin promoter factor 1, homeodomain transcription factor; islet/duodenum homeobox 1; pancreatic and duodenal homeobox gene 1; somatostatin-transactivating factor 1
• Gene ID: 18609
• mRNA Refseq: NM_008814.3
• Protein Refseq: NP_032840.1
• UniProt ID: P52946

Reviews

08/03/2022

The expression was better at high humidity.

06/18/2022

It is stable in clinical trials.

06/01/2021

It has stable expression in signal transduction.

Q&As

How to study the regulatory mechanism of PDX1?

Studying the regulatory mechanism of PDX1 requires a combination of various experimental methods and techniques, such as gene knockout, transcriptome analysis, and protein-protein interactions.

Do abnormal PDX1 levels suggest some disease?

Abnormal PDX1 levels may indicate pancreas-related diseases such as diabetes, pancreatitis, and pancreatic cancer.

How are mutations in PDX1 detected and analyzed?

Mutations in PDX1 can be detected and analyzed by methods such as whole-genome sequencing or target region sequencing to understand the impact of mutations on protein structure and function.

What are the mutation types of PDX1?

The types of mutations in PDX1 include point mutations, insertions/deletions, duplications, etc., which may cause structural and functional abnormalities of the protein.

Is there a relationship between PDX1 and other genes or proteins?

PDX1 has a complex relationship with other genes or proteins, and can interact with other genes or proteins and participate in a variety of biochemical reactions.

What are the applications of PDX1 in medical treatment?

It can be used as a biomarker in medical treatment to help monitor disease progression and treatment efficacy. In addition, therapeutics targeting PDX1, such as gene therapy or modulating its expression, are also being studied.