Recombinant Mouse Rspo1, His tagged
Cat.No. : | Rspo1-4024M |
Product Overview : | Recombinant Mouse Rspo1 (NP_619624.2) full length (Met 1-Gln 265), fused with a polyhistidine tag at the C-terminus, was produced in Human Cell. |
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Source : | Human Cells |
Species : | Mouse |
Tag : | His |
Form : | Lyophilized from sterile PBS, pH 7.4 |
Molecular Mass : | The secreted recombinant mouse RSPO1 comprises 256 amino acids with a predicted molecular mass of 28.5 kDa. As a result of glycosylation, the apparent molecular mass of the protein is approximately 43 kDa in SDS-PAGE under reducing conditions. |
Endotoxin : | < 1.0 eu per μg of the protein as determined by the LAL method. |
Stability : | Samples are stable for up to twelve months from date of receipt at -70oC. |
Storage : | Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Gene Name : | Rspo1 R-spondin homolog (Xenopus laevis) [ Mus musculus ] |
Official Symbol : | Rspo1 |
Gene ID : | 192199 |
mRNA Refseq : | NM_138683 |
Protein Refseq : | NP_619624 |
Products Types
◆ Recombinant Protein | ||
RSPO1-132H | Active Recombinant Human RSPO1 Protein (Ser21-Ala263), N-His-SUMO tagged, Animal-free, Carrier-free | +Inquiry |
RSPO1-34H | Recombinant Human RSPO1 Protein | +Inquiry |
RSPO1-422H | Recombinant Human RSPO1 Protein, His-tagged | +Inquiry |
RSPO1-28H | Active Recombinant Human RSPO1 Protein, Pre-aliquoted | +Inquiry |
RSPO1-421H | Recombinant Human RSPO1 Protein, His-tagged | +Inquiry |
◆ Lysates | ||
RSPO1-001MCL | Recombinant Mouse RSPO1 cell lysate | +Inquiry |
RSPO1-1918HCL | Recombinant Human RSPO1 cell lysate | +Inquiry |
RSPO1-001HCL | Recombinant Human RSPO1 cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionRSPO1 stands out for its potential therapeutic use in Regenerative Medicine due to its mitogenic activity in stem cells.
Characterization methods included Western blotting, mass spectrometry, and in vitro (C2C12 cells) and in vivo (BALB/c mice) biological activity assays, confirming structural integrity and biological efficacy.
RSPOs regulate various tissue-specific processes, including bone formation, skeletal muscle tissue development, proliferation of pancreatic β-cells, intestinal stem cells, and cancer progression.
The purification strategy for rhRSPO1 involved two sequential chromatographic steps: heparin affinity chromatography followed by molecular exclusion chromatography.
RSPO1 induces the Wnt pathway by stabilizing the Wnt receptor complex, leading to enhanced Wnt signaling and influencing cellular processes.
rhRSPO1 was generated using the HEK293 cell line, and the coding sequence was synthesized and subcloned into a mammalian cell expression vector.
R-Spondin proteins, including RSPO1, play crucial roles in cell proliferation, differentiation, and death by inducing the Wnt pathway.
Customer Reviews (3)
Write a reviewRemarkable changes in cell behavior were attributed to the potent effects of the product.
The product's respected reputation in the scientific community was a testament to its quality and effectiveness.
Dependable protein-protein interaction outcomes were achieved due to the product's pure composition.
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