Recombinant Rat Acan protein, His-tagged

• Cat.No.: Acan-1957R
• Product Overview: Recombinant Rat Acan aa. (Pro34~Glu147 (Accession # P07897)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Source: E. coli
• Species: Rat
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 14 kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Pro34~Glu147 (Accession # P07897)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: > 95%
• Characteristic: The isoelectric point is 6.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in PBS, pH7.4.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Acan aggrecan [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Acan
• Synonyms: HIF1B; TANGO; bHLHe2; HIF1BETA; HIF-1beta; HIF1-beta; HIF-1-beta; class E basic helix-loop-helix protein 2; dioxin receptor, nuclear translocator; hypoxia-inducible factor 1, beta subunit; aryl hydrocarbon receptor nuclear translocator
• Gene ID: 58968
• mRNA Refseq: NM_022190.1
• Protein Refseq: NP_071526.1
• UniProt ID: P07897

Reviews

08/12/2022

The technical support team at Creative BioMart went above and beyond to customize the ACAN protein to my specific requirements. Their expertise and professionalism were greatly appreciated.

06/13/2020

I was thrilled to find the recombinant ACAN protein on sale at such an affordable price.

02/14/2020

I highly recommend Creative BioMart to anyone in need of recombinant proteins. Their products are top-notch and their customer service is exceptional.

Q&As

What are some challenges in studying ACAN protein and osteoarthritis?

One major challenge is the complex nature of the disease, which involves multiple factors and pathways. Additionally, the high heterogeneity of cartilage tissue and the difficulty in obtaining samples pose challenges for experimental studies. Another challenge is the limited understanding of the molecular mechanisms underlying the degradation and synthesis of ACAN protein and its interactions with other matrix molecules and cell surface receptors.

What are the benefits of highly purified recombinant protein?

A high purity level of recombinant protein is desirable as it ensures the protein's integrity and reduces the risk of impurities or contaminants that may affect its function.

What is the function of ACAN protein in cartilage?

ACAN protein is a major component of the extracellular matrix of cartilage and plays a critical role in maintaining its structural integrity. ACAN protein helps to maintain the hydration and swelling properties of cartilage by binding to water molecules through its GAG chains. It also interacts with other matrix molecules and cell surface receptors, which contributes to the mechanical properties of cartilage.

What is the structure of ACAN protein?

ACAN protein is a large proteoglycan consisting of a core protein and long chains of glycosaminoglycans (GAGs), primarily chondroitin sulfate and keratan sulfate. The core protein is composed of several domains, including a signal peptide, a globular domain, and multiple tandem repeats of a hyaluronan-binding domain (HABD).

How to determine the purity level of recombinant ACAN protein?

The purity level of recombinant protein is typically determined through a combination of different techniques, such as SDS-PAGE, HPLC, and mass spectrometry. The purity level of the protein can vary depending on the production method and the quality control measures in place.

What applications is recombinant ACAN protein suitable for?

Recombinant ACAN protein is suitable for a variety of applications, including biochemical assays, biophysical studies, and structural biology. The protein can also be used in cell culture experiments and in vitro assays to study its interaction with other proteins and molecules. Additionally, recombinant ACAN protein may have potential applications in tissue engineering and regenerative medicine, where it can be used to create biomaterials for cartilage repair and regeneration.

What is the endotoxin level of recombinant ACAN protein, and how is it tested?

The endotoxin level of recombinant protein is typically tested using the Limulus amebocyte lysate (LAL) assay. Endotoxins are a type of bacterial cell wall component that can contaminate protein samples, and their presence can affect the function of the protein. A low endotoxin level is desirable for recombinant ACAN protein as it ensures the protein's purity and reduces the risk of contamination. You can select specific recombinant protein endotoxin levels in the inquiry form.

What is the recommended concentration of recombinant ACAN protein for use in cell culture experiments?

The recommended concentration of recombinant ACAN protein for use in cell culture experiments may vary depending on the specific experimental conditions and cell type used. Typically, a concentration range of 10-100 μg/mL is used.

What are some potential therapeutic strategies targeting ACAN protein for osteoarthritis?

One potential therapeutic strategy is to use molecules that inhibit the degradation of ACAN protein, such as aggrecanase inhibitors. Another approach is to enhance the synthesis of ACAN protein or its GAG chains, which could be achieved by gene therapy or the use of growth factors. Additionally, tissue engineering approaches that aim to regenerate cartilage by using ACAN protein and other matrix molecules have also been explored.

How does ACAN protein contribute to the development of osteoarthritis?

During the development of osteoarthritis, ACAN protein is degraded by various enzymes, including matrix metalloproteinases and aggrecanases. This degradation results in a loss of cartilage integrity, decreased hydration and swelling properties, and altered mechanical properties of cartilage, which contribute to the development of osteoarthritis.