Recombinant Rat ARFGAP2 Protein Pre-coupled Magnetic Beads

• Cat.No.: ARFGAP2-410R-B
• Product Overview: The Recombnant protein was conjugated to magnetic beads. This ready-to-use, pre-coupled magnetic beads are in uniform particle size and narrow size distribution with large surface area, which is conducive to convenient and fast capture target molecules with high specificity and achieve magnetic separation. This product can be equipped with automation equipment for high-throughput operations.

Specification

• Source: HEK293
• Species: Rat
• Form: Solution
• Particle size: ~2 μm
• Beads Surface: Hydrophilic
• Capacity: > 200 pmol rabbit IgG/ mg beads
• Applications: Immunoassay, In vitro diagnostics, cell sorting, Immunoprecipitation/Co-precipitation, Protein/antibody separation and purification.
• Stability: Stable for at least 6 months from the date of receipt of the product under proper storage and handling conditions.
• Storage: 2-8℃. Do not to freeze thaw the Beads
• Concentration: 10mg beads/mL
• Storage Buffer: PBS buffer

Gene Information

• Gene Name: Arfgap2 ADP-ribosylation factor GTPase activating protein 2 [ Rattus norvegicus ]
• Official Symbol: ARFGAP2
• Gene ID: 362162
• mRNA Refseq: NM_001033707.1
• Protein Refseq: NP_001028879.1
• UniProt ID: Q3MID3

Reviews

08/05/2022

the ARFGAP2 protein offers numerous advantages in trials and research experiments.

10/14/2021

Whether in Western blotting, immunoprecipitation, or other applications, the ARFGAP2 Protein consistently delivers outstanding performance.

01/27/2020

Highly recommended for its exceptional performance in ELISA, the ARFGAP2 protein is a top contender for researchers involved in protein electron microscopy structure analysis.

06/21/2019

Meeting my experimental needs with utmost precision, it has surpassed my expectations.

04/03/2019

Their expertise and dedication have greatly assisted in solving problems and optimizing experimental protocols.

09/08/2018

I am thrilled to express my satisfaction with the ARFGAP2 Protein, as it has consistently proven to be of outstanding quality and entirely suited to meet my experimental needs.

07/11/2018

Coupled with the manufacturer's exceptional technical support and commitment to customer satisfaction, researchers can confidently utilize the ARGI protein in their studies, knowing that they have the necessary resources and assistance to succeed.

05/16/2018

Not only does it provide reliable and consistent results due to its high quality and purity, but it also holds significant value in understanding cellular processes and disease mechanisms.

Q&As

What are the known functions of ARFGAP2?

ARFGAP2 is primarily involved in regulating membrane trafficking processes within cells. It functions as a GTPase-activating protein (GAP) for the ADP-ribosylation factor 1 (ARF1) protein, which is essential for vesicle formation and trafficking between different cellular compartments. By activating ARF1, ARFGAP2 helps in coordinating the sorting, packaging, and delivery of proteins and lipids to their appropriate destinations. Additionally, ARFGAP2 has been implicated in the regulation of lipid metabolism, cell motility, endocytosis, and exocytosis.

Can ARFGAP2 be involved in signal transduction pathways?

While ARFGAP2 is primarily linked to membrane trafficking processes, there is emerging evidence suggesting that it can also participate in signaling pathways. For example, ARFGAP2 has been shown to interact with and modulate the activity of certain kinases, indicating a potential role in signal transduction. However, further research is needed to elucidate the exact mechanisms and significance of its involvement in signaling.

Are there any known ARFGAP2 isoforms?

Yes, alternative splicing of the ARFGAP2 gene produces different isoforms. At least three isoforms of ARFGAP2 have been identified, which differ in their protein length and specific domains. These isoforms may have distinct functions or subcellular localizations.

In which cellular processes is ARFGAP2 involved?

ARFGAP2 is involved in multiple cellular processes such as vesicle trafficking, protein sorting, and membrane remodeling. It plays a crucial role in the formation and regulation of coat protein complex I (COPI) vesicles, which facilitate retrograde transport from the Golgi apparatus to the endoplasmic reticulum.

Are there any known binding partners of ARFGAP2?

ARFGAP2 interacts with multiple proteins to carry out its various functions. Some of its known binding partners include ARF1, phospholipase D1 (PLD1), and certain kinases such as protein kinase D (PKD) and p21-activated kinase 1 (PAK1). These interactions are critical for coordinating membrane trafficking events and the regulation of cellular processes.

Is ARFGAP2 expression regulated in a tissue-specific manner?

Yes, studies have shown that ARFGAP2 expression can vary in different tissues and cell types. For example, higher levels of ARFGAP2 expression have been reported in the brain and testis compared to other tissues. Moreover, its expression levels can also change under different physiological and pathological conditions.

Are there any animal models used to study ARFGAP2?

Yes, animal models such as mice have been used to study the function and physiological significance of ARFGAP2. By creating knockout or transgenic mice with altered ARFGAP2 expression, researchers can investigate its role in development, behavior, and disease progression.

Are there any interacting partners or binding partners of ARFGAP2?

Yes, ARFGAP2 has been shown to interact with multiple proteins. For instance, it interacts with ARF proteins, facilitating their GTPase activity. ARFGAP2 also interacts with components of the COPI vesicle machinery, such as coatomer subunits, helping in vesicle formation. Furthermore, ARFGAP2 has been reported to interact with other regulatory proteins involved in membrane trafficking, including ARFGAP1 and clathrin, among others.

Can ARFGAP2 be targeted for therapeutic purposes?

Given its involvement in various diseases, ARFGAP2 could be a potential therapeutic target. However, further research is needed to better understand the specific roles of ARFGAP2 in disease progression and identify strategies to modulate its activity. Targeting ARFGAP2 could potentially impact membrane trafficking, which is a fundamental cellular process, making it a challenging target to manipulate selectively.

Are there any specific diseases associated with ARFGAP2 mutations?

Yes, mutations in the ARFGAP2 gene have been linked to certain diseases. For example, mutations in ARFGAP2 have been found in individuals with intellectual disability, suggesting a potential role in neurodevelopmental disorders. Additionally, dysregulation of ARFGAP2 expression has been observed in some cancers and may play a role in tumor growth and progression.