Recombinant Rat UCHL3 protein(Glu2-Ala230), His-tagged

• Cat.No.: Uchl3-1810R
• Product Overview: Recombinant Rat UCHL3 (Q91Y78) (Glu 2-Ala 230) was expressed in E. coli, fused with a polyhistidine tag at the N-terminus.

Specification

• Source: E. coli
• Species: Rat
• Tag: N-His
• Protein length: Glu2-Ala230
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by the hydrolysis of UbiquitinAMC. The specific activity is >14000 pmoles/min/μg.
• Molecular Mass: The recombinant rat UCHL3 comprises 240 amino acids and predicts a molecular mass of 27.5 kDa. The apparent molecular mass of the rat UCHL3 is approximately 28 kDa in SDS-PAGE under reducing conditions.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Uchl3 ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) [ Rattus norvegicus ]
• Official Symbol: UCHL3
• Synonyms: UCHL3; ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase); ubiquitin carboxyl-terminal hydrolase isozyme L3; UCH-L3; ubiquitin thioesterase L3; RGD1561196
• Gene ID: 498560
• mRNA Refseq: NM_001110165
• Protein Refseq: NP_001103635