| Description : |
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. |
| Source : |
E.coli |
| Species : |
Rhesus |
| Form : |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. |
| Bio-activity : |
Fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using human HeLa cells infected with encephalomyocarditis (EMC) virus is less than 20.0 ng/ml, corresponding to a specific activity of > 5.0×10⁴ IU/mg. |
| Molecular Mass : |
Approximately 16.8 kDa, a single non-glycosylated polypeptide chain containing 142 amino acids. |
| Protein length : |
142 |
| AA Sequence : |
QDPYVKEAENLKKYFNAGDPDVADN GTLFLDILRNWKEESDRKIMQSQIV SFYFKLFKNFKDDQRIQKSVETIKE DINVKFFNSNKKKRDDFEKLTNYSV TDSNVQRKAVHELIQVMAELSPAAK IGKRKRSQMFRGRRASQ |
| Endotoxin : |
Less than 0.1 EU/µg of rRhIFN-γ as determined by LAL method. |
| Purity : |
>97% by SDS-PAGE and HPLC analyses. |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
| Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
