Recombinant S. pyogenes Cas9 (H840A) Protein, His-tagged
Cat.No. : | Cas9-05S |
Product Overview : | Recombinant full length SpCas9 (H840A)-NLS from S. pyogenes was expressed in E. coli using a C-terminal His tag. |
- Specification
- Gene Information
- Related Products
Description : | Cas9 is a multidomain nuclease from the bacterium S. pyogenes characteristic of type II CRISPR- Cas systems. Using single guide RNA (sgRNA), Cas9 is targeted to specific genomic loci where it generates a blunt ended double strand beak. SpCas9 (H840A)-NLS is a catalytic mutant of Cas9 containing a nuclear localization sequence for optimal activity. Unlike wild type Cas9, SpCas9(H840A)-NLS generates a single strand DNA break on the non-targeting strand. SpCas9 (H840A)-NLS can be used for various genome editing applications. |
Source : | E. coli |
Species : | S. pyogenes |
Tag : | His |
Purity : | >70% |
Stability : | One year at -70 centigrade from deta of shipment. |
Storage : | Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles. Always include RNase inhibitor at manufacturer recommended concentrations in Cas enzyme reactions. |
Concentration : | 0.2 μg/μL 6.26 pmol/μg |
Storage Buffer : | Recombinant protein stored in 50mM sodium phosphate, pH 7.5, 300mM NaCl, 200mM imidazole, 1mM DTT, 10% glycerol. |
Shipping : | On dry ice. |
Official Symbol : | Cas9 |
Synonyms : | Cas9; Csn1; Spy_1046; Cas9-H840A Nickase |
Protein Refseq : | AAK33936 |
UniProt ID : | Q99ZW2 |
Products Types
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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