Recombinant Yeast AHA1, His-tagged

• Cat.No.: AHA1-30Y
• Product Overview: Recombinant Yeast AHA1, fused with His tag, was expressed in E. coli.

Specification

• Description: Aha1 is a member of the Hsp90 cochaperone family, and is thought to stimulate Hsp90 ATPase activity by competing with p23 and other co-chaperones for Hsp90 binding. It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/Hsp90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors.
• Source: E. coli
• Species: Yeast
• Tag: His
• Form: 12.5mM Hepes buffer pH7.2, 10mM NaCl, 50% glycerol
• Molecular Mass: ~40.5kDa
• Purity: >90% pure by SDS - PAGE analysis.
• Applications: WB Control, functional/activity studies
• Storage: -20°C; 1 year+ Avoid freeze/ thaw cycle.

Reviews

05/09/2022

Using this recombination protein was like having a secret weapon in the lab – it's that good!

10/01/2021

Hands down, the best protein I've ever worked with. I'm a fan!

01/19/2020

I love using this recombination protein in my research. It's so easy to work with!

03/24/2019

As a researcher, I rely on high-quality product, and this one exceeded my expectations.

03/02/2018

I'm so glad I discovered this protein; it has simplified my experiments immensely.

Q&As

What are the implications of AHA1 dysregulation in diseases?

AHA1 dysfunction has been associated with cancer, neurodegenerative disorders, and other diseases where Hsp90 client proteins play critical roles.

What are the molecular mechanisms underlying AHA1's role in regulating signal transduction pathways?

AHA1 can modulate the conformation and activity of specific signaling proteins, affecting downstream signaling events.

How does AHA1 affect the stability of oncogenic client proteins in cancer cells?

AHA1 can stabilize and promote the maturation of oncogenic client proteins, contributing to cancer cell survival and proliferation.

Describe the role of AHA1 in cellular responses to environmental stressors.

AHA1 is involved in stress response pathways, assisting in the folding and stabilization of stress-responsive proteins.

How does AHA1 facilitate the ATPase activity of Hsp90?

AHA1 binds to the middle domain of Hsp90 and stimulates its ATPase activity, accelerating the ATP hydrolysis necessary for chaperone function.

What is the role of AHA1 in protein folding and quality control mechanisms?

AHA1 assists in the correct folding of Hsp90 client proteins and also participates in the recognition and degradation of misfolded proteins.

How is the AHA1 gene transcriptionally regulated?

Transcription factors, co-activators, and epigenetic modifications can control the expression of the AHA1 gene under different cellular conditions.

How is the expression of AHA1 regulated under stress conditions?

AHA1 expression can be upregulated by various stress-inducing factors, such as heat shock, oxidative stress, or exposure to toxic compounds.

Discuss the cellular localization of AHA1 and its implications.

AHA1 is primarily localized in the cytoplasm, but it can shuttle between the cytoplasm and nucleus, influencing diverse cellular processes.

Describe the post-translational modifications that modulate AHA1 activity.

Phosphorylation and acetylation are known to regulate AHA1, influencing its interaction with Hsp90 and client proteins.