Recombinant Zebrafish ACTA1A

• Cat.No.: ACTA1A-2499Z
• Product Overview: Recombinant Zebrafish ACTA1A full length or partial length protein was expressed.

Specification

• Source: Mammalian Cells
• Species: Zebrafish
• Tag: His
• Form: Liquid or lyophilized powder
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >80%
• Notes: This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
• Storage: Store it at +4 oC for short term. For long term storage, store it at -20 oC～-80 oC.
• Storage Buffer: PBS buffer

Gene Information

• Gene Name: acta1a actin, alpha 1a, skeletal muscle [ Danio rerio (zebrafish) ]
• Official Symbol: ACTA1A
• Gene ID: 550445
• mRNA Refseq: NM_001017750
• Protein Refseq: NP_001017750
• UniProt ID: Q567E8

Reviews

12/14/2022

The protein product's prolonged stability provided me with peace of mind, knowing that I could rely on it whenever I needed

09/04/2022

The protein product's precision in delivering consistent results across replicates further validated its quality and suitability for my research

08/09/2021

I felt confident in using the protein product, knowing that it underwent rigorous safety testing and met the highest industry standards

10/22/2020

The protein product's compatibility with commonly used laboratory equipment and techniques simplified its integration into my existing workflow

09/23/2017

The protein product's compatibility with diverse experimental techniques ensured its versatility and applicability across different research areas

Q&As

What is the primary role of alpha actins within the body?

Alpha actins serve as a major constituent of the contractile apparatus in muscle tissues.

In which tissues are alpha actins predominantly found?

Alpha actins are predominantly found in muscle tissues.

Are there any other significant roles or functions associated with actin isoforms?

Actin isoforms, including alpha, beta, and gamma, have various additional roles in processes such as cell shape maintenance, cell division, endocytosis, and cell signaling.

How do beta and gamma actins differ from alpha actins in terms of their distribution within cells?

Beta and gamma actins coexist in most cell types as components of the cytoskeleton, whereas alpha actins are primarily found in muscle tissues.

Are there any known interactions between the ACTA1a protein and others?

Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others.

How do the transgenic zebrafish models contribute to understanding ACTA1a-related nemaline myopathy?

The transgenic zebrafish models expressing mutant forms of ACTA1a help researchers study the molecular defects underlying ACTA1-related nemaline myopathy. By observing the resulting myopathic phenotypes, they gain insights into the disease mechanisms and potential therapeutic targets.

List some variants of ACTA1a.

There are variants like acta1a H42Y, M134V and V165M.

What family does ACTA1a protein belong to?

It belongs to actin family.

What are the three main groups of actin isoforms found in vertebrates?

 The three main groups of actin isoforms found in vertebrates are alpha, beta, and gamma actins.

What is ACTA1a?

ACTA1a is a gene that codes for the skeletal muscle actin protein in zebrafish. It is the equivalent gene to human ACTA1, which is involved in muscle contraction.

What is the role of ACTA1a in muscle function?

ACTA1a plays a crucial role in muscle contraction by forming part of the actin filaments, which are essential for generating the force required for muscle movement.

Can you provide an example of how beta and gamma actins contribute to internal cell motility?

Beta and gamma actins play a role in processes such as cell migration, organelle movement, and cytoplasmic streaming within cells.

What are the functions of beta and gamma actins in most cell types?

Beta and gamma actins function as mediators of internal cell motility within most cell types.

What function is related to the transcriptional levels of acta1a?

Heart development and skeletal muscle formation are related to the transcriptional levels of acta1a.

Where are acta1a expressed?

ACTA1a is the predominant actin expressed in fast myofibers.

What were the observed phenotypes in the transgenic zebrafish with ACTA1a mutations?

The fish expressing mutant actins displayed myopathic phenotypes, including delayed hatching from the chorion and variable degrees of curved bodies. They also exhibited muscle weakness, thin bodies, curved tails, reduced motility, actin aggregates, Z-line thickening, and severe myofibrillar disorganization.

What is the subcellular Location of ACTA1a protein?

It located in cytoplasm and cytoskeleton.