Recombinant Zebrafish AMBRA1A

• Cat.No.: AMBRA1A-8356Z
• Product Overview: Recombinant Zebrafish AMBRA1A full length or partial length protein was expressed.

Specification

• Source: Mammalian Cells
• Species: Zebrafish
• Tag: His
• Form: Liquid or lyophilized powder
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >80%
• Notes: This item requires custom production and lead time is between 5-9 weeks. We can custom produce according to your specifications.
• Storage: Store it at +4 oC for short term. For long term storage, store it at -20 oC～-80 oC.
• Storage Buffer: PBS buffer

Gene Information

• Gene Name: ambra1a autophagy/beclin-1 regulator 1a [ Danio rerio (zebrafish) ]
• Official Symbol: AMBRA1A
• Gene ID: 100332642
• mRNA Refseq: NM_001281992
• Protein Refseq: NP_001268921
• UniProt ID: L0N9K3

Reviews

10/27/2022

Enhanced multiplexing capability for simultaneous analysis of multiple proteins.

11/07/2020

Compatibility with various cell culture systems for functional studies.

09/04/2018

Accurate determination of protein-protein interaction stoichiometry.

Q&As

How does AMBRA1A interact with other autophagy regulators, such as ATG proteins and Beclin 1?

AMBRA1A interacts with ATG proteins and Beclin 1 to form protein complexes that regulate autophagy initiation and autophagosome formation.

What are the specific cargoes targeted by AMBRA1A-mediated autophagy and their implications in cellular homeostasis?

AMBRA1A targets specific cargoes for degradation via autophagy, including protein aggregates, damaged organelles, and intracellular pathogens.

What are the therapeutic implications of targeting AMBRA1A in autophagy-related diseases, such as cancer and neurodegenerative disorders?

Targeting AMBRA1A in autophagy-related diseases holds therapeutic potential, as modulating its activity may restore autophagic flux and mitigate pathological processes in cancer and neurodegenerative disorders.

What is the impact of AMBRA1A knockout on embryonic development and tissue homeostasis?

AMBRA1A knockout leads to embryonic lethality and disrupted tissue homeostasis, highlighting its crucial role in development and cellular maintenance.

How does AMBRA1A contribute to the formation and turnover of autophagosomes during autophagy flux?

AMBRA1A plays a critical role in autophagosome formation and turnover by coordinating the nucleation, elongation, and closure steps of autophagy.

What is the role of AMBRA1A in the regulation of neuronal autophagy and neurodegenerative diseases?

AMBRA1A is involved in neuronal autophagy, and dysregulation of its function is implicated in neurodegenerative diseases, such as Alzheimer's and Parkinson's.

What are the downstream effectors and signaling pathways regulated by AMBRA1A in autophagy induction?

AMBRA1A regulates downstream effectors and signaling pathways, such as mTORC1 and AMPK, to control autophagy induction and cellular responses to stress.

How does post-translational modification of AMBRA1A, such as phosphorylation or ubiquitination, affect its function in autophagy?

Post-translational modifications of AMBRA1A, like phosphorylation or ubiquitination, modulate its activity and interactions with other autophagy-related proteins.

What are the structural domains of AMBRA1A that mediate its interactions with other proteins in the autophagy machinery?

The structural domains of AMBRA1A, including its WD40 repeats and coiled-coil domains, mediate interactions with other proteins in the autophagy machinery.

How does AMBRA1A influence the balance between autophagy and apoptosis in response to cellular stress?

AMBRA1A maintains the balance between autophagy and apoptosis, contributing to cell survival or cell death decisions under various stress conditions.