Recombinant mouse ADAM15 (O88839-1) extracellular domain (Met 1-Thr 697) was expressed, with a C-terminal polyhistidine tag.
Predicted N Terminal:
Arg 18 & Asp 208
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
1. Measured by its ability to bind human PTK6 in a functional ELISA. 2. Measured by its ability to bind human SRC in a functional ELISA. 3. Measured by its ability to bind mouse SRC in a functional ELISA. 4. Measured by its ability to bind human LYN in a
The secreted recombinant mouse ADAM15 (pro form) comprises 691 amino acids and has a calculated molecular mass of 74.6 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 80-90 kDa in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>90 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.