Recombinant Mouse Il17b protein, Fc-tagged

• Cat.No.: Il17b-440M
• Product Overview: Recombinant Mouse Il17b(Met1-Phe180) fused with the Fc region of human IgG1 at the C-terminus was expressed in Insect cells.

Specification

• Source: Insect cells
• Species: Mouse
• Tag: Fc
• Predicted N Terminal: Arg 23
• Form: Lyophilized from sterile 100 mM Glycine, 10 mM NaCl, pH 7.4.1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.2. Please contact us for any concerns or special requirements.
• Molecular Mass: The recombinant mouse IL17B/Fc is a disulfide-linked homodimer. The reduced monomer comprises 396 amino acids and has a predicted molecular mass of 44.5 kDa. The apparent molecular mass of the protein is approximately 57 kDa in SDS-PAGE under reducing conditions.
• Protein length: Met1-Phe180
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: Il17b interleukin 17B [ Mus musculus ]
• Official Symbol: Il17b
• Synonyms: IL17B; interleukin 17B; interleukin-17B; IL-17B; cytokine CX1; cytokine-like protein ZCYTO7; gene for cytokine-like protein; neuronal interleukin-17-related factor; Zcyto7; 1110006O16Rik; 1700006N07Rik
• Gene ID: 56069
• mRNA Refseq: NM_019508
• Protein Refseq: NP_062381
• Chromosome Location: 18; 18 D3
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem
• Function: cytokine activity; receptor binding

Reviews

05/14/2022

Suitable for signaling studies.

10/30/2020

High solubility

10/10/2017

Potent in B-cell assays.

Q&As

What strategies are employed to investigate the cross-talk between IL17B and other cytokines in inflammatory responses?

Co-culture experiments and signaling pathway analysis reveal IL17B's interaction with other cytokines.

How does IL17B's structure affect its receptor binding affinity, and what experimental techniques reveal this?

Techniques like surface plasmon resonance elucidate IL17B's binding to its receptor.

How is the impact of IL17B on cancer progression studied using in vivo models?

In vivo models combine IL17B overexpression or neutralization with tumor models to study its role.

What experimental designs are used to understand the role of IL17B in tissue-specific inflammation in human samples?

Human tissue samples are analyzed using immunohistochemistry and cytokine profiling to understand IL17B's effects.

Can you elaborate on the techniques used to determine IL17B's role in modulating immune cell behavior?

Flow cytometry and functional assays explore how IL17B affects immune cell activation and cytokine production.

What are the approaches to analyzing IL17B's involvement in neuroinflammation and central nervous system disorders?

Research uses animal models and biomarker analysis to study IL17B in neuroinflammatory diseases.

Can you explain the use of genetic knockout or knockdown techniques to validate IL17B's functional effects?

Genetic techniques like CRISPR/Cas9 knockout or siRNA knockdown validate IL17B's functional impact.

Could you detail the experimental protocols to assess the effect of IL17B on endothelial barrier function?

Transwell assays and impedance-based methods evaluate IL17B's impact on endothelial barrier integrity.

How do researchers identify the downstream signaling pathways activated by IL17B and its receptor?

Western blotting and phosphorylation assays uncover IL17B's downstream signaling pathways.

What experimental methods differentiate IL17B from other IL17 family members during gene expression analysis?

Gene expression analysis involves qPCR or RNA sequencing to distinguish IL17B expression patterns.