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Home > Products > Recombinant Proteins > Recombinant Rat Wee1 protein, GST-tagged, active

Recombinant Rat Wee1 protein, GST-tagged, active

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GST(3)
His(4)
MYC/DDK(1)
Cat.No.:
Wee1-32R
Product Overview:
Recombinant Rat Wee1 fused with GST tag at N-terminal was expressed in E. coli.
Description:
Protein inhibitor of Cdk2 kinase. Wee1 is a protein tyrosine kinase that phosphorylates Cdc2 on Tyr15 and hence inactivates the CDK1 and CDK2, which facilitates in the negative regulation of entry of cells into mitosis. Increased level of wee1 stimulates Human Immunodeficiency Virus type 1 (HIV-1) Vpr induced cell cycle arrest at G2/M transition followed by apoptosis.
Source:
E. coli
Species:
Rat
Tag :
GST
Form:
Solution in 50 mM Tris-HCl, pH 7.5, 1 mM EDTA, 1 mM DTT, 10% Glycerol
Molecular Mass:
~110 kDa
Unit Definition:
One unit of Wee 1 activity is defined as the amount of enzyme that produces 90% inhibition of the activity of 10 units of CDK2. One unit of CDK2 will transfer 1 pmol of phosphate to histone H1 per minute at pH 7.2 and 30 centigrade.
Applications:
Wee1, active from rat may be used to study apoptotic signaling.
Storage:
Recombinant rat Wee 1 is stable for at least six months when stored at -70 centigrade. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Avoid repeated freeze-thaw cycles. Do not store in a frost-free freezer.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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