Active GMP Recombinant Human IL15 Protein
Cat.No. : | IL15-336HG |
Product Overview : | Recombinant human IL15 (NP_000576.1) (Asn49-Ser162) was expressed in an animal component free process under cGMP guidelines. |
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Source : | E. coli |
Species : | Human |
Predicted N Terminal : | Asn 49 |
Form : | Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5 % mannitol and 0.01% Tween80. |
Bio-activity : | Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 2-8 ng/mL. The specific activity of Recombinant Human IL-15 is approximately 2.4×10^5 units/μg. |
Molecular Mass : | The GMP recombinant human IL15 consists of 114 amino acids and predicts a molecular mass of 12.8 kDa. |
Endotoxin : | < 0.01 EU per μg of the protein as determined by the LAL method. |
Purity : | > 95 % as determined by SDS-PAGE. |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Shipping : | In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. |
Gene Name : | IL15 interleukin 15 [ Homo sapiens ] |
Official Symbol : | IL15 |
Synonyms : | IL15; interleukin 15; interleukin-15; IL 15; MGC9721; IL-15; |
Gene ID : | 3600 |
mRNA Refseq : | NM_000585 |
Protein Refseq : | NP_000576 |
MIM : | 600554 |
UniProt ID : | P40933 |
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◆ Lysates | ||
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionTechniques discern IL15 and IL2 receptor binding kinetics.
Methods explore IL15's role in autoimmune disease mechanisms.
IL15 knockout models provide insights into its diverse functions.
IL15's influence on memory T cell differentiation investigated.
Immunofluorescence microscopy visualizes IL15 subcellular localization.
Approaches validate IL15's involvement in inflammatory responses.
IL15's role in NK cell cytotoxicity analyzed using assays.
ELISA measures IL15 secretion accurately.
qPCR quantifies IL15's impact on regulatory T cells.
Flow cytometry quantifies IL15-driven T-cell proliferation.
Customer Reviews (3)
Write a reviewMapping protein- interactions in DNA transcription for gene regulation.
Suitable for cytotoxicity assays.
Effective in NK cell activation.
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