Active Recombinant Cynomolgus/Mouse IL23A&IL12B protein, His-tagged

• Cat.No.: IL23A&IL12B-1612C
• Product Overview: Recombinant Cynomolgus/Mouse IL23A&IL12B protein(Val22-Pro189(IL23A)&Met23-Ser335(IL12B)), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Cynomolgus/Mouse
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.
• Bio-activity: Immobilized Biotinylated Human IL-23 R, Fc,Avitag at 1 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate can bind Cynomolgus IL-23A&Mouse IL-12B Heterodimer Protein, His Tag&Tag Free with a linear range of 0.03-0.25 μg/mL.
• Molecular Mass: Cynomolgus IL-23A&Mouse IL-12B Heterodimer Protein, His Tag&Tag Free, produced by co-expression of IL-23 alpha and IL-12 beta, has a calculated MW of 20.3 kDa (IL-23 alpha) and 35.8 kDa (IL-12 beta). Subunit IL-23 alpha is fused with a polyhistidine tag at the N-terminus and subunit IL-12 beta contains no tag. The reducing (R) protein migrates as 20-23 kDa (IL-23 alpha) and 40-42 kDa and 43-46 kDa (IL-12 beta) respectively due to glycosylation.
• Proteinlength: Val22-Pro189(IL23A)&Met23-Ser335(IL12B)
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Reviews

04/18/2020

Coupled with the manufacturer's exceptional technical support and commitment to customer satisfaction, researchers can confidently utilize the IL23A protein in their studies, knowing that they have the necessary resources and assistance to succeed.

12/02/2016

Its remarkable attributes and consistent results make it an indispensable tool in various experimental applications.

02/25/2016

Highly recommended for its exceptional performance in ELISA, the IL23A protein is a top contender for researchers involved in protein electron microscopy structure analysis.

Q&As

Is this bioactive cyno IL-23 heterodimer (p19/p40) or just the cyno IL-23p19 subunit?

Please inquiry our product manager with specific product.

Are there any FDA-approved drugs targeting IL23A?

Yes, drugs like ustekinumab and risankizumab, which target IL23A, have been approved for certain autoimmune diseases.

Is IL23A involved in the regulation of adaptive or innate immunity?

IL23A primarily plays a role in regulating the adaptive immune response.

How is IL23A linked to the pathogenesis of ankylosing spondylitis?

IL23A is associated with the inflammation of the spine and joints seen in ankylosing spondylitis, making it a target for therapeutic intervention.

How does IL23A contribute to rheumatoid arthritis?

In rheumatoid arthritis, IL23A is involved in the dysregulation of the immune system, leading to joint inflammation and damage.

How is IL23A involved in inflammatory bowel diseases like Crohn's disease?

IL23A is implicated in the pathogenesis of Crohn's disease, contributing to chronic inflammation in the gastrointestinal tract.