Active Recombinant Feline IL15

• Cat.No.: IL15-1099F
• Product Overview: Recombinant Feline IL15 (Accession # O97687) Asn49-Ser162, fused with , was produced in E. coli.

Specification

• Source: E. coli
• Species: Feline
• Predicted N Terminal: Asn49
• Form: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
• Bio-activity: Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359.The ED50 for this effect is typically 0.2-1.2 ng/mL.
• Molecular Mass: Recombinant Feline IL15 has a calculated MW of 13 kDa. In SDS-PAGE migrates as 10 kDa, reducing conditions.
• Protein length: Asn49-Ser162
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: IL15 interleukin 15 [ Felis catus (domestic cat) ]
• Official Symbol: IL15
• Synonyms: interleukin-15; interleukin-15; IL-15
• Gene ID: 493682
• mRNA Refseq: NM_001009207.1
• Protein Refseq: NP_001009207.1
• UniProt ID: O97687
• Chromosome Location: chromosome: B1

Reviews

04/19/2018

Mapping protein- interactions in DNA transcription for gene regulation.

02/20/2018

Suitable for cytotoxicity assays.

10/22/2017

Effective in NK cell activation.

Q&As

What techniques differentiate IL15 and IL2 receptor binding kinetics?

Techniques discern IL15 and IL2 receptor binding kinetics.

What experimental methods study IL15's involvement in autoimmune diseases?

Methods explore IL15's role in autoimmune disease mechanisms.

What are the applications of IL15 knockout models in deciphering its functions?

IL15 knockout models provide insights into its diverse functions.

How do you analyze IL15's impact on memory T cell differentiation?

IL15's influence on memory T cell differentiation investigated.

How does immunofluorescence microscopy visualize IL15 localization in cells?

Immunofluorescence microscopy visualizes IL15 subcellular localization.

What approaches validate IL15's role in inflammatory responses?

Approaches validate IL15's involvement in inflammatory responses.

Could you explain the role of IL15 in NK cell cytotoxicity assays?

IL15's role in NK cell cytotoxicity analyzed using assays.

Can you detail the use of ELISA in quantifying IL15 secretion?

ELISA measures IL15 secretion accurately.

How is IL15's effect on regulatory T cells quantified using qPCR?

qPCR quantifies IL15's impact on regulatory T cells.

How does flow cytometry assess IL15-induced T-cell proliferation?

Flow cytometry quantifies IL15-driven T-cell proliferation.