Active Recombinant Human ADAM10, His-tagged

• Cat.No.: ADAM10-18H
• Product Overview: Recombinant Human ADAM10(Thr214-Glu672), fused with a C-terminal 10-His tag, was expressed in Sf 21(baculovirus).

Specification

• Description: Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin.
• Source: Sf 21 (baculovirus)
• Species: Human
• Tag: His
• Predicted N Terminal: Thr214
• Form: Recombinant Human ADAM10 is prone to proteolytic cleavage at C-terminus. The predominant form of the purified protein lacks the His tag. Supplied as a 0.2 µm filtered solution in MES, NaCl, ZnCl2,Glycerol and Brij-35.
• Bio-activity: Measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2.
• Molecular Mass: Predicted Molecular Mass:52 kDaSDS-PAGE:60 kDa, reducing conditions
• Endotoxin: < 1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles.6 months from date of receipt, -70 °C as supplied.3 months, -70 °C under sterile conditions after opening.

Gene Information

• Gene Name: ADAM10 ADAM metallopeptidase domain 10 [ Homo sapiens ]
• Official Symbol: ADAM10
• Synonyms: ADAM10; ADAM metallopeptidase domain 10; a disintegrin and metalloproteinase domain 10; disintegrin and metalloproteinase domain-containing protein 10; CD156c; HsT18717; kuz; MADM
• Gene ID: 102
• mRNA Refseq: NM_001110
• Protein Refseq: NP_001101
• MIM: 602192
• UniProt ID: O14672
• Chromosome Location: 15q22
• Pathway: Activated NOTCH1 Transmits Signal to the Nucleus; Collagen degradation; Constitutive Signaling by NOTCH1 PEST Domain Mutants
• Function: SH3 domain binding; endopeptidase activity; metalloendopeptidase activity

Reviews

09/16/2021

During the experiment, the technician helped me solve the problems in a timely and accurate manner.

04/03/2021

The ADAM10 proteins purchased from your company is suitable for WB.

09/08/2020

Received high quality protein products and obtained satisfactory experimental results.

Q&As

What proteins does ADAM10 cleave?

ADAM10 protein can cleave TNF-α and E-cadherin, etc.

Does adding GST tag have any effect on protein?

The addition of the GST tag helps protect the recombinant protein from extracellular protease degradation and improves its stability.

How to distinguish between ADAM10 precursors and active bodies?

The protein size of ADAM10 precursor is larger than that of ADAM10 active.

In addition to cardiovascular tumor-related research, ADAM10 is currently used in the research of which diseases?

ADAD1 is currently also used in the research of Alzheimer's disease and diabetic retinopathy, etc.