Recombinant Human ADAM10 protein, His-tagged

• Cat.No.: ADAM10-7638H
• Product Overview: Recombinant Human ADAM10 aa. (Arg420~Val641 (Accession # O14672)) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing.
• Source: E. coli
• Species: Human
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 25.7kDa
• Protein length: Arg420~Val641 (Accession # O14672)
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 6.5.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: ADAM10 ADAM metallopeptidase domain 10 [ Homo sapiens (human) ]
• Official Symbol: ADAM10
• Synonyms: RAK; kuz; AD10; AD18; MADM; CD156c; CDw156; HsT18717; a disintegrin and metalloprotease domain 10; kuzbanian protein homolog; mammalian disintegrin-metalloprotease; disintegrin and metalloproteinase domain-containing protein 10
• Gene ID: 102
• mRNA Refseq: NM_001110.3
• Protein Refseq: NP_001101.1
• UniProt ID: O14672

Reviews

09/16/2021

During the experiment, the technician helped me solve the problems in a timely and accurate manner.

04/03/2021

The ADAM10 proteins purchased from your company is suitable for WB.

09/08/2020

Received high quality protein products and obtained satisfactory experimental results.

Q&As

What proteins does ADAM10 cleave?

ADAM10 protein can cleave TNF-α and E-cadherin, etc.

Does adding GST tag have any effect on protein?

The addition of the GST tag helps protect the recombinant protein from extracellular protease degradation and improves its stability.

How to distinguish between ADAM10 precursors and active bodies?

The protein size of ADAM10 precursor is larger than that of ADAM10 active.

In addition to cardiovascular tumor-related research, ADAM10 is currently used in the research of which diseases?

ADAD1 is currently also used in the research of Alzheimer's disease and diabetic retinopathy, etc.