Active Recombinant Human ADAM10 Protein, His-tagged

• Cat.No.: ADAM10-924H
• Product Overview: Recombinant Human ADAM10 (Accession # O14672) Thr214-Glu672, fused with a C-terminal 10-His tag, was produced in Spodoptera frugiperda, Sf 21 (baculovirus)-derived.

Specification

• Source: Baculovirus Sf21 Insect Cells
• Species: Human
• Tag: His
• Predicted N Terminal: Thr214
• Form: Supplied as a 0.2 µm filtered solution in MES, NaCl, ZnCl2,Glycerol and Brij-35.
• Bio-activity: Measured by its ability to cleave a fluorogenic peptide substrate Mca-KPLGL-Dpa-AR-NH2.The specific activity is >20 pmol/min/µg, as measured under the described conditions.
• Molecular Mass: Recombinant Human ADAM10, His-tagged has a calculated MW of 52 kDa. In SDS-PAGE migrates as 60 kDa, reducing conditions.
• Protein length: Thr214-Glu672
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: ADAM10 ADAM metallopeptidase domain 10 [ Homo sapiens ]
• Official Symbol: ADAM10
• Synonyms: ADAM10; ADAM metallopeptidase domain 10; a disintegrin and metalloproteinase domain 10; disintegrin and metalloproteinase domain-containing protein 10; CD156c; HsT18717; kuz; MADM; CDw156; ADAM 10; kuzbanian protein homolog; mammalian disintegrin-metalloprotease; a disintegrin and metalloprotease domain 10; AD10
• Gene ID: 102
• mRNA Refseq: NM_001110
• Protein Refseq: NP_001101
• MIM: 602192
• UniProt ID: O14672
• Chromosome Location: 15q2

Reviews

09/16/2021

During the experiment, the technician helped me solve the problems in a timely and accurate manner.

04/03/2021

The ADAM10 proteins purchased from your company is suitable for WB.

09/08/2020

Received high quality protein products and obtained satisfactory experimental results.

Q&As

What proteins does ADAM10 cleave?

ADAM10 protein can cleave TNF-α and E-cadherin, etc.

Does adding GST tag have any effect on protein?

The addition of the GST tag helps protect the recombinant protein from extracellular protease degradation and improves its stability.

How to distinguish between ADAM10 precursors and active bodies?

The protein size of ADAM10 precursor is larger than that of ADAM10 active.

In addition to cardiovascular tumor-related research, ADAM10 is currently used in the research of which diseases?

ADAD1 is currently also used in the research of Alzheimer's disease and diabetic retinopathy, etc.