Active Recombinant Human CSF1R(538-end) Protein, N-His-tagged

• Cat.No.: CSF1R-91H
• Product Overview: N-terminal 6Histagged, recombinant, human FMS, amino acids 538–end, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTAagarose.

Specification

• Description: CSF1R (colony stimulating factor I receptor), formerly known as FMS (McDonough feline sarcoma viral oncogene), is a receptor tyrosine kinase for CSF-1 and expressed primarily in monocytes, macrophages, and human tumor tissue. CSF-1 binding to FMS induces dimerization of the tyrosine kinase domain of the receptor, and stimulates the survival, proliferation, and differentiation of hematopoietic cells of the monocyte-macrophage series.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 538-end
• Form: 1.282mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: this lot showed a minimum activity of 317U/mg, where one unit of FMS activity is defined as 1nmol phosphate incorporated into 250μM (KKKSPGEYVNIEFG) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 50.2 kDa
• AA Sequence: 1 MHHHHHHEFY KYKQKPKYQV RWKIIESYEG NSYTFIDPTQ LPYNEKWEFP RNNLQFGKTL 61 GAGAFGKVVE ATAFGLGKED AVLKVAVKML KSTAHADEKE ALMSELKIMS HLGQHENIVN 121 LLGACTHGGP VLVITEYCCY GDLLNFLRRK AEAMLGPSLS PGQDPEGGVD YKNIHLEKKY 181 VRRDSGFSSQ GVDTYVEMRP VSTSSNDSFS EQDLDKEDGR PLELRDLLHF SSQVAQGMAF 241 LASKNCIHRD VAARNVLLTN GHVAKIGDFG LARDIMNDSN YIVKGNARLP VKWMAPESIF 301 DCVYTVQSDV WSYGILLWEI FSLGLNPYPG ILVNSKFYKL VKDGYQMAQP AFAPKNIYSI 361 MQACWALEPT HRPTFQQICS FLQEQAQEDR RERDYTNLPS SSRSGGSGSS SSELEEESSS 421 EHLTCCEQGD IAQPLLQPNN YQFC
• Purity: Purity 94.1% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: CSF1R colony stimulating factor 1 receptor [ Homo sapiens (human) ]
• Official Symbol: CSF1R
• Synonyms: CSF1R; colony stimulating factor 1 receptor; FMS; CSFR; FIM2; GPSC; HDLS; C-FMS; CD115; HDLS1; CSF-1R; BANDDOS; M-CSF-R
• Gene ID: 1436
• mRNA Refseq: NM_005211.4
• Protein Refseq: NP_005202.2
• MIM: 164770
• UniProt ID: P07333