Active Recombinant Human DUSP4 Protein, His-tagged

• Cat.No.: DUSP4-106H
• Product Overview: Recombinant human MKP-2 fused at the N-terminus to a His-tag was produced in Sf9 insect cells.

Specification

• Description: The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1, ERK2 and JNK, is expressed in a variety of tissues, and is localized in the nucleus. Two alternatively spliced transcript variants, encoding distinct isoforms, have been observed for this gene. In addition, multiple polyadenylation sites have been reported.
• Source: Insects cell
• Species: Human
• Tag: His
• Form: Liquid
• Bio-activity: ≥60 U/mg assayed by 3-O-methyl fluorescein phosphate (OMFP) hydrolysis at pH 8.2, 30 centigrade. One unit is equal to 1 nmole phosphate hydrolyzed from OMFP per minute.
• Molecular Mass: 43 kDa
• Storage: Store at -80 centigrade.
• Storage Buffer: In 20mM TRIS/HCl, pH 8.0, 200mM NaCl, 5mM DTT, 0.1 % Tween-20, 10% glycerol.
• Shipping: Dry ice

Gene Information

• Gene Name: DUSP4 dual specificity phosphatase 4 [ Homo sapiens (human) ]
• Official Symbol: DUSP4
• Synonyms: DUSP4; dual specificity phosphatase 4; TYP; HVH2; MKP2; MKP-2; dual specificity protein phosphatase 4; MAP kinase phosphatase 2; VH1 homologous phosphatase 2; dual specificity protein phosphatase hVH2; mitogen-activated protein kinase phosphatase 2; serine/threonine specific protein phosphatase; EC 3.1.3.16; EC 3.1.3.48
• Gene ID: 1846
• mRNA Refseq: NM_001394
• Protein Refseq: NP_001385
• MIM: 602747
• UniProt ID: Q13115

Reviews

08/16/2022

The protein composition was stable and concentrated, allowing me to obtain satisfactory experimental results.

07/21/2022

Very good after-sales service to get my experiments going!

02/04/2022

The technician modified my experimental protocol so that my experiment went smoothly.

Q&As

How is DUSP4 involved in the signaling pathway?

DUSP4 inactivates MAPKs by dephosphorylating them and thus performs an important function in the MAPK signaling pathway

What is DUSP4 also known as?

DUSP4 also known as mitogen-activated protein kinase phosphatase 2

Which membrane is recommended for DUSP4 protein for WB?

You can choose PVDF membrane.

What diseases is DUSP4 involved in?

DUSP4 is involved in diseases such as laryngeal, breast and colorectal cancer

What are the effects of overexpression of DUSP4?

In colon cancer, overexpression of DUSP4 may lead to increased proliferation of tumor cells through stimulation of growth-promoting genes such as Myc and cell cycle protein D1