Recombinant Human DUSP4 293 Cell Lysate

• Cat.No.: DUSP4-6773HCL

Specification

• Description: Antigen standard for dual specificity phosphatase 4 (DUSP4), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: DUSP4 dual specificity phosphatase 4 [ Homo sapiens ]
• Official Symbol: DUSP4
• Synonyms: DUSP4; dual specificity phosphatase 4; dual specificity protein phosphatase 4; HVH2; MAP kinase phosphatase 2; MKP 2; TYP; VH1 homologous phosphatase 2; dual specificity protein phosphatase hVH2; serine/threonine specific protein phosphatase; mitogen-activated protein kinase phosphatase 2; MKP2; MKP-2
• Gene ID: 1846
• mRNA Refseq: NM_057158
• Protein Refseq: NP_476499
• MIM: 602747
• UniProt ID: Q13115
• Chromosome Location: 8p12-p11
• Pathway: Activated TLR4 signalling, organism-specific biosystem; B Cell Receptor Signaling Pathway, organism-specific biosystem; ERK/MAPK targets, organism-specific biosystem; ERKs are inactivated, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; MAP kinase activation in TLR cascade, organism-specific biosystem
• Function: MAP kinase tyrosine/serine/threonine phosphatase activity; hydrolase activity; protein tyrosine phosphatase activity; protein tyrosine/threonine phosphatase activity

Reviews

08/16/2022

The protein composition was stable and concentrated, allowing me to obtain satisfactory experimental results.

07/21/2022

Very good after-sales service to get my experiments going!

02/04/2022

The technician modified my experimental protocol so that my experiment went smoothly.

Q&As

How is DUSP4 involved in the signaling pathway?

DUSP4 inactivates MAPKs by dephosphorylating them and thus performs an important function in the MAPK signaling pathway

What is DUSP4 also known as?

DUSP4 also known as mitogen-activated protein kinase phosphatase 2

Which membrane is recommended for DUSP4 protein for WB?

You can choose PVDF membrane.

What diseases is DUSP4 involved in?

DUSP4 is involved in diseases such as laryngeal, breast and colorectal cancer

What are the effects of overexpression of DUSP4?

In colon cancer, overexpression of DUSP4 may lead to increased proliferation of tumor cells through stimulation of growth-promoting genes such as Myc and cell cycle protein D1