Active Recombinant Human Retinoid X Receptor, Beta

• Cat.No.: RXRB-1153H

Specification

• Cat. No.: RXRB-1153H
• Description: Retinoid X Receptor (RXR) serves as a promiscuous heterodimerization partner for many nuclear receptors through the identity box, a 40-amino acid subregion within the ligand binding domain. The human RXRβ, gene has been mapped to the short arm or centromeric region of chromosome 6 (6pter-q13) which also contains the MHC I locus. Two major RXRβ isoforms in the mouse, designated RXRβ1 and RXRβ2 (GenBank accession numbers D21830 and D21831), differ in the N-terminal (A) domain with RXRβ 1 containing an extra 72 amino acids. These isoform mRNAs are transcribed from two CpG island promoters and the different N-terminal exons are spliced to exons common to both isoforms from the remainder of the gene. Although the two mouse RXR isoforms have been characterized in some detail, less information is available about their human counterparts. Since the expression of different RXR isoforms appears to be well conserved between species, each receptor isoform may hold a specific role in retinoid signalling. However, many RXR expression studies have not recognised the importance of establishing which RXR isoforms are expressed in a particular system. RXRβ is predominantly involved in retinoid responses in the N-type SH SY 5Y and the S-type SH S EP neuroblastoma cells, both derivatives of a mixed phenotype neuroblastoma cell line. Recombinant RXR is isolated from an E.coli strain that carries the coding sequence of the human RXRβ under the control of a T7 promoter.
• Source: Baculovirus.
• Form: Liquid. Supplied in 20mM Tris-HCl pH8.0, 100mM KCl, 0.2mM EDTA, 1mM DTT and 20% glycerol.
• Activity: 20ng are sufficient for an in vitro transcription assay and 100ng are sufficient for a protein-protein interaction assay.
• Application: RXRβ has been applied in in vitro transcription assays, DNA and protein-protein interaction assays.
• Purity: >95% by SDS-PAGE.
• Usage: For in vitro use only.
• Storage: Quality guaranteed for 12 months, store at -80℃. Avoid freeze / thaw cycles.

Gene Information

• Gene Name: RXRB retinoid X receptor, beta [ Homo sapiens ]
• Synonyms: RXRB; retinoid X receptor, beta; NR2B2; DAUDI6; RCoR-1; MGC1831; H-2RIIBP; MHC class I promoter binding protein; Retinoid X receptor beta; Nuclear receptor subfamily 2 group B member 2
• Gene ID: 6257
• mRNA Refseq: NM_021976
• Protein Refseq: NP_068811
• MIM: 180246
• UniProt ID: P28702
• Chromosome Location: 6p21.3
• Pathway: Adipocytokine signaling pathway; Non-small cell lung cancer; PPAR signaling pathway; Pathways in cancer; Small cell lung cancer; Thyroid cancer
• Function: ligand-regulated transcription factor activity; metal ion binding; retinoid-X receptor activity; sequence-specific DNA binding; steroid binding; steroid hormone receptor activity; transcription coactivator activity; transcription factor activity; zinc ion binding

Reviews

10/19/2022

Timely support for protein expression optimization, appreciated assistance.

04/08/2021

High-quality protein characterization, fundamental for our research.

05/25/2018

Accurate protein structural analysis, indispensable for our investigations.

Q&As

How do environmental factors like diet and sunlight exposure affect the activity and expression of RXRB?

Environmental factors like diet and sunlight alter RXRB activity, impacting its role in skin health and metabolic processes.

What are the effects of RXRB dysregulation on cancer development and progression?

Dysregulation of RXRB can contribute to the development and progression of various cancers.

How does RXRB influence the immune response, especially in autoimmune and inflammatory diseases?

RXRB modulates the immune system, affecting autoimmune and inflammatory responses.

How does RXRB contribute to metabolic regulation, particularly in lipid metabolism and obesity?

RXRB is crucial in lipid metabolism, influencing processes related to obesity and metabolic health.

What role does RXRB play in skin health and the development of skin disorders?

RXRB plays a key role in maintaining skin health and is involved in the pathology of skin disorders.

What is the impact of RXRB on neurodevelopment and neurodegenerative diseases?

RXRB impacts neurodevelopment and is implicated in the progression of neurodegenerative diseases.

How does RXRB interact with retinoic acid and other ligands to regulate gene expression?

RXRB binds retinoic acid and other ligands to regulate genes involved in development, metabolism, and cell differentiation.