Active Recombinant Human RSPO3 protein, Fc-tagged

• Cat.No.: RSPO3-0654H
• Product Overview: Recombinant Human RSPO3 protein(Gln22-His272), fused with Fc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Fc
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human R-Spondin 3 Protein, Fc Tag at 5 μg/mL (100 μL/well) can bind Human LGR4, His Tag with a linear range of 0.02-0.625 μg/mL.
• Molecular Mass: This protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 54.9 kDa. The protein migrates as 55-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Gln22-His272
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: RSPO3 R-spondin 3 [ Homo sapiens ]
• Official Symbol: RSPO3
• Synonyms: RSPO3; R-spondin 3; R spondin 3 homolog (Xenopus laevis) , thrombospondin, type I, domain containing 2 , THSD2; R-spondin-3; FLJ14440; R-spondin 3 homolog; roof plate-specific spondin-3; protein with TSP type-1 repeat; thrombospondin, type I, domain containing 2; thrombospondin type-1 domain-containing protein 2; PWTSR; THSD2; CRISTIN1
• Gene ID: 84870
• mRNA Refseq: NM_032784
• Protein Refseq: NP_116173
• MIM: 610574
• UniProt ID: Q9BXY4

Reviews

07/24/2022

Dependable partner, a vital research collaborator.

09/09/2021

Consistency in quality, invaluable for our experiments.

03/22/2020

Reliable data source, accelerates our research progress.

Q&As

How do genetic variations in RSPO3 affect developmental processes?

Genetic mutations in RSPO3 can disrupt normal development and tissue regeneration.

What potential therapeutic applications arise from targeting RSPO3 in regenerative medicine and cancer?

Targeting RSPO3 could offer advances in regenerative medicine and cancer therapy by modulating Wnt signaling.

What role does RSPO3 play in stem cell biology?

RSPO3 plays a significant role in maintaining stem cell populations and influencing their differentiation.

How does RSPO3 contribute to tissue development and regeneration?

It stimulates tissue development and regeneration by activating Wnt pathways, essential for cellular proliferation.

What is the primary role of RSPO3 in Wnt signaling?

RSPO3, a member of the R-spondin family, enhances Wnt signaling, crucial for cellular growth and differentiation.

What is the impact of altered RSPO3 activity on cancer progression?

Altered RSPO3 activity is linked to cancer development and progression, particularly in colorectal cancer.

How does RSPO3 interact with other components in Wnt signaling pathways?

RSPO3 interacts synergistically with Wnt pathway components, amplifying signal transduction.