Recombinant Human RSPO3 cell lysate

• Cat.No.: RSPO3-1906HCL
• Product Overview: Human RSPO3 / R-spondin 3 (aa 1-146) derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Human cells
• Species: Human
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Gene Information

• Gene Name: RSPO3 R-spondin 3 [ Homo sapiens ]
• Official Symbol: RSPO3
• Synonyms: RSPO3; R-spondin 3; R spondin 3 homolog (Xenopus laevis) , thrombospondin, type I, domain containing 2 , THSD2; R-spondin-3; FLJ14440; R-spondin 3 homolog; roof plate-specific spondin-3; protein with TSP type-1 repeat; thrombospondin, type I, domain containing 2; thrombospondin type-1 domain-containing protein 2; PWTSR; THSD2; CRISTIN1
• Gene ID: 84870
• mRNA Refseq: NM_032784
• Protein Refseq: NP_116173
• MIM: 610574
• UniProt ID: Q9BXY4
• Chromosome Location: 6q22.33
• Function: frizzled binding; heparin binding; receptor binding

Reviews

07/24/2022

Dependable partner, a vital research collaborator.

09/09/2021

Consistency in quality, invaluable for our experiments.

03/22/2020

Reliable data source, accelerates our research progress.

Q&As

How do genetic variations in RSPO3 affect developmental processes?

Genetic mutations in RSPO3 can disrupt normal development and tissue regeneration.

What potential therapeutic applications arise from targeting RSPO3 in regenerative medicine and cancer?

Targeting RSPO3 could offer advances in regenerative medicine and cancer therapy by modulating Wnt signaling.

What role does RSPO3 play in stem cell biology?

RSPO3 plays a significant role in maintaining stem cell populations and influencing their differentiation.

How does RSPO3 contribute to tissue development and regeneration?

It stimulates tissue development and regeneration by activating Wnt pathways, essential for cellular proliferation.

What is the primary role of RSPO3 in Wnt signaling?

RSPO3, a member of the R-spondin family, enhances Wnt signaling, crucial for cellular growth and differentiation.

What is the impact of altered RSPO3 activity on cancer progression?

Altered RSPO3 activity is linked to cancer development and progression, particularly in colorectal cancer.

How does RSPO3 interact with other components in Wnt signaling pathways?

RSPO3 interacts synergistically with Wnt pathway components, amplifying signal transduction.