Description : |
IL-1β is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1α and IL-1β binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1β is a secreted cytokine, IL-1α is predominantly a cell-associated cytokine. |
Source : |
E. coli |
Species : |
Mouse |
Form : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Bio-activity : |
The ED50 as determined by the dose-dependent stimulation of murine D10S cells is < 0.002 ng/mL, corresponding to a specific activity of > 5 × 10^8 units/mg. |
Molecular Mass : |
Approximately 17.5kDa, a single non-glycosylated polypeptide chain containing 153 amino acids. |
Protein Length : |
153 |
AA Sequence : |
MVPIRQLHYRLRDEQQKSLVLSDPY ELKALHLNGQNINQQVIFSMSFVQG EPSNDKIPVALGLKGKNLYLSCVMK DGTPTLQLESVDPKQYPKKKMEKRF VFNKIEVKSKVEFESAEFPNWYIST SQAEHKPVFLGNNSGQDIIDFTMES VSS |
Endotoxin : |
Less than 1 EU/mg of rmIL-1β as determined by LAL method. |
Purity : |
>98% by SDS-PAGE and HPLC analyses. |
Storage : |
This lyophilized preparation is stable at 2-8 centigrade, but should be kept at -20 centigrade for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 centigrade. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 to -70 centigrade. Avoid repeated freeze/thaw cycles. |
Storage Buffer : |
Lyophilized from a 0.2mm filtered solution in PBS, pH 7.4. |
Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at < -20 centigrade. Further dilutions should be made in appropriate buffered solutions. |