Active Recombinant Mouse Interleukin 15, His-tagged

• Cat.No.: Il15-739M
• Product Overview: Recombinant mouse Il15, fused to His-tagged, was expressed in E. coli and purified by using conventional chromatography after refolding of the isolated inclusion bodies in a redox buffer. MW: 17.6 kDa.

Specification

• Description: Il15 is a pleiotropic cytokine that plays a pivotal role in both innate and adaptive immunity and has structural similarity to IL2 that is secreted by mononuclear phagocytes following infection by viruses. Stimulation by Il15 requires interaction of Il15 with components of IL-2R, including IL-2Rbeta and probably IL-2R gamma, but not IL-2R alpha.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Liquid. In Phosphate Buffered Saline (pH7.4) containing 10% glycerol.
• Bio-activity: Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 for this effect is< 1>
• Molecular Mass: 17.6 kDa (152aa), confirmed by MALDI-TOF.
• Protein length: 49-162aa
• AA Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMNW IDVRYDLEKI ESLIQSIHID TTLYTDSDFH PSCKVTAMNC FLLELQVILH EYSNMTLNET VRNVLYLANS TLSSNKNVAE SGCKECEELE EKTFTEFLQS FIRIVQMFIN TS
• Endotoxin: < 1.0 eu per 1 μg of protein (determined by lal
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: Il15 interleukin 15 [ Mus musculus ]
• Official Symbol: Il15
• Synonyms: IL15; interleukin 15; interleukin-15; IL-15; AI503618
• Gene ID: 16168
• mRNA Refseq: NM_001254747
• Protein Refseq: NP_001241676
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Cytokines and Inflammatory Response (BioCarta), organism-specific biosystem; HTLV-I infection, organism-specific biosystem; HTLV-I infection, conserved biosystem; Herpes simplex infection, organism-specific biosystem; Herpes simplex infection, conserved biosystem
• Function: cytokine activity; cytokine activity; cytokine receptor binding

Reviews

04/19/2018

Mapping protein- interactions in DNA transcription for gene regulation.

02/20/2018

Suitable for cytotoxicity assays.

10/22/2017

Effective in NK cell activation.

Q&As

What techniques differentiate IL15 and IL2 receptor binding kinetics?

Techniques discern IL15 and IL2 receptor binding kinetics.

What experimental methods study IL15's involvement in autoimmune diseases?

Methods explore IL15's role in autoimmune disease mechanisms.

What are the applications of IL15 knockout models in deciphering its functions?

IL15 knockout models provide insights into its diverse functions.

How do you analyze IL15's impact on memory T cell differentiation?

IL15's influence on memory T cell differentiation investigated.

How does immunofluorescence microscopy visualize IL15 localization in cells?

Immunofluorescence microscopy visualizes IL15 subcellular localization.

What approaches validate IL15's role in inflammatory responses?

Approaches validate IL15's involvement in inflammatory responses.

Could you explain the role of IL15 in NK cell cytotoxicity assays?

IL15's role in NK cell cytotoxicity analyzed using assays.

Can you detail the use of ELISA in quantifying IL15 secretion?

ELISA measures IL15 secretion accurately.

How is IL15's effect on regulatory T cells quantified using qPCR?

qPCR quantifies IL15's impact on regulatory T cells.

How does flow cytometry assess IL15-induced T-cell proliferation?

Flow cytometry quantifies IL15-driven T-cell proliferation.