Active Recombinant Human ADAM Metallopeptidase Domain 8, His-tagged

• Cat.No.: ADAM8-525H
• Product Overview: Recombinant Human ADAM8protein was expressed in Murine myeloma cell line with a C-terminal 6-His tag.Met1-Pro497.

Specification

• Cat. No.: ADAM8-525H
• Description: ADAM8, also known ascell surface antigen MS2 or CD156a, is a member of the ADAM family that containsa disintegrin and metalloproteaselike domain. ADAM8 can cleave a variety of substratesand has been shown as a sheddase for the low affinity IgE receptor CD23 andthe neural recognition molecule CHL1. Expression and regulation studiessuggest that ADAM8 is a novel osteoclast stimulating factor and may play arole in asthma.
• Source: Mammalian cells
• Form: Supplied as a 0.2 μmfiltered solution in Glycerol, Tris, NaCl and CaCl2.
• N-terminalSequence: Glu158
• Molecular Weight: 38 kDa
• Activity: Measuredby its ability to cleave a fluorogenic peptide substrate Mca-PLAQAV-DpaRSSSR-NH2,The specific activity is > 1 pmol/min/μg, as measured under the described conditions.
• Endotoxin Level: < 1.0 EU per 1 μgof the protein by the LAL method.
• SDS-PAGE: 42 kDa, reducingconditions.
• Purity: > 90%, by SDSPAGEunder reducing conditions and visualized by silver stain.
• Storage: Use a manual defrostfreezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt,-20 to -70°C as supplied. 3 months, -20 to -70°C under sterile conditionsafter opening.

Gene Information

• Gene Name: ADAM8 ADAM metallopeptidasedomain 8 [ Homo sapiens ]
• Official Symbol: ADAM8
• Synonyms: ADAM8; ADAMmetallopeptidase domain 8; MS2; CD156; CD156a; disintegrin andmetalloproteinase domain-containing protein 8; cell surface antigen MS2;human leukocyte differentiation antigen; a disintegrin and metalloproteinasedomain 8; EC 3.4.24.-
• Gene ID: 101
• mRNA Refseq: NM_001109
• Protein Refseq: NP_001100
• MIM: 602267
• UniProt ID: P78325
• Chromosome Location: 10q26.3
• Function: calcium ion binding;cell adhesion molecule binding; metalloendopeptidase activity;metallopeptidase activity; peptidase activity; protein binding; protein self-association;zinc ion binding

Reviews

02/08/2022

The ADAM8 protein received was of stable composition and high concentration, giving me satisfactory experimental results.

06/22/2021

It is suitable for ELISA, and the help of technicians also made my experiments go smoothly.

06/09/2021

The technician gave me a detailed introduction to the ADAM38 protein for the smooth running of the subsequent experiments.

07/03/2020

The after-sales service of the product is guaranteed, which has helped me solve many problems in the experiment.

07/18/2019

Good protein quality, good service attitude and affordable price.

Q&As

What physiological processes is ADAM8 involved in?

ADAM8 is involved in various complex biological processes such as inflammation and osteoclastogenesis.

My research has relatively high requirements for protein purity. Do you provide high-purity ADAM8 in stock?

You can choose the product whose Cat.No. is ADAM8-086H.

Can ADAM8 cleave proteins?

ADAM8 cleaves important cell surface proteins, such as cytokines and growth factors.

Is the ADAM8 in stock?

We provide a variety of ADAM8 proteins, all of which are in stock except those that require custom production.

Which of HIS and GST has less effect on ADAM8 protein?

His has a smaller molecular weight and has less impact on protein activity.

Is the precursor ADAM8 protein form active?

No, ADAM8 can only be activated by removing the leading domain.

Does propofol have an effect on the expression of ADAM8 in pancreatic cancer cells?

Propofol inhibits ADAM8 expression in pancreatic cancer cells.

If I customize ADAM8 with special requirements, can I specify the expression system?

Yes, you can first communicate with our experts about specific customization requirements.

What is the expression level of ADAM8 under normal conditions?

The expression level of ADAM8 is usually low in normal tissues and is significantly increased in response to pathological stimuli.

How soon will the customized ADAM8 arrive?

Lead times for custom-manufactured recombinant proteins range from 5-9 weeks.

Is SP1 combined with ADAM8?

Yes, transcription factor SP1 binds to the promoter of ADAM8.

What are the two subtypes of ADAM8?

ADAM8 has two subtypes, the membrane type (ADAM8m) and the secretory type (ADAM8s).

In which diseases is ADAM8 currently used in research?

ADAM8 is mainly used in the studies of breast, prostate and pancreatic cancers.

Can I specify a product that has two product forms?

Yes, we will ship according to your specification after your purchase.