Rabbit Monoclonal Antibody to Human Serpin Peptidase Inhibitor, Clade A, Member 3

• Cat.No.: CAB11598RH
• Product Overview: Rabbit Monoclonal Antibody to Human Serpin Peptidase Inhibitor, Clade A, Member 3

Specification

• Antigen Description: Human SerpinA3, also known as Alpha 1-antichymotrypsin (AACT), is a plasma alpha globulin glycoprotein, and is a member of serpin superfamily of the serine protease inhibitors consisting of at least 35 members. SerpinA3 has been demonstrated to inhibit the activity of certain serine proteases, such as cathepsin G found in neutrophils, and chymases present in mast cells, by inducing a major conformational rearrangement, and thus protects some tissues from damage caused by proteolytic enzymes. This enzyme is produced primarily in the liver, and is identified as an acute-phase inflammatory protein. SerpinA3 deficiency has been associated with liver disease, and mutations of this gene have been observed in patients with Parkinson disease and chronic obstructive pulmonary disease. In addition, ACT gene polymorphism has been implicated with Alzheimer's disease (AD), cerebral amyloid angiopathy (CAA), as well as stroke, since SerpinA3 is a major constituent of the plaques in AD and an inhibitor of amyloid beta peptide degradation.
• Specificity: Human SerpinA3. No cross-reactivity in ELISA with Human SerpinA7; Human SerpinA5; Human SerpinC1; Human SerpinD1; Human SerpinF2; Human cell lysate (293 cell line)
• Immunogen: Recombinant Human SerpinA3 Protein
• Host: Rabbit
• Isotype: Rabbit IgG
• Species: Human
• Clone: A007
• Applications: Western blot; ELISA
• Dilution: Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human SERPINA3 in WB. Using a DAB detection system, the detection limit for Human SERPINA3 is approximately 10 ng/lane under non-reducing conditions and reducing conditions.Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human SERPINA3. The detection limit for Human SERPINA3 is approximately 0.0195 ng/well.
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Human SerpinA3
• Format: 0.2 μm filtered solution in PBS with 5% trehalose
• Storage: This antibody can be stored at 2-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 to -70 °C. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: SERPINA3 serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3 [ Homo sapiens ]
• Official Symbol: SERPINA3
• Synonyms: SERPINA3; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3; AACT,alpha 1 antichymotrypsin ,serine (or cysteine) proteinase inhibitor, clade A (alpha 1 antiproteinase, antitrypsin), member 3; alpha-1-antichymotrypsin; ACT; alpha 1 antichymotrypsin; serpin A3; growth-inhibiting protein 24; growth-inhibiting protein 25; cell growth-inhibiting gene 24/25 protein; serine (or cysteine) proteinase inhibitor, clade A, member 3; AACT; GIG24; GIG25; MGC88254
• Gene ID: 12
• mRNA Refseq: NM_001085
• Protein Refseq: NP_001076
• MIM: 107280
• UniProt ID: P01011
• Chromosome Location: 14q32.1
• Pathway: Selenium Pathway, organism-specific biosystem
• Function: DNA binding; peptidase inhibitor activity; protein binding; serine-type endopeptidase inhibitor activity; serine-type endopeptidase inhibitor activity

Reviews

03/14/2022

The product's impact extended to data analysis, with intuitive tools for interpreting experimental results.

06/14/2020

The longevity of the product's effects allowed us to study cellular responses over extended timeframes.

10/31/2017

We found that the product's support for data analysis and visualization significantly expedited our research.

Q&As

What are the physiological roles of Serpina3n?

Serpina3n plays significant roles in various physiological processes, including modulation of the complement cascade, apoptosis, wound healing, and potential implications in Alzheimer's disease.

What is the significance of Serpina3n in pathologies?

Recent studies have reported significant roles of Serpina3n during various pathologies, particularly in central nervous system (CNS) insults, making it a potential therapeutic target.

What is the aim of literature drawing together Serpina3n applications?

The aim is to compile literature shedding light on the potential therapeutic applications of Serpina3n/SERPINA3 in various diseases. Additionally, a brief comparison between murine Serpina3n and its human ortholog SERPINA3 (α1-antichymotrypsin) is provided to account for their biological roles and significance.

Are the full range of Serpina3n applications known?

The full range of potential applications of Serpina3n is yet to be fully revealed, but its reported implications, especially in CNS insults, suggest potential therapeutic applications.

What is Serpina3n?

Serpina3n is a secretory serine protease inhibitor belonging to clade "a" with unique structural and physiological characteristics. |

How does Serpina3n inhibit proteases?

Serpina3n inhibits a wide range of proteases, contributing to its diverse physiological roles.

To which clade does Serpina3n belong?

Serpina3n belongs to clade "a" of serine protease inhibitors. |