Recombinant Human AFP, StrepII-tagged
Cat.No. : | AFP-225H |
Product Overview : | Purified, full-length human recombinant Alpha-fetoprotein or aFP protein (amino acids 20-609, 590 a.a.) with StrepII tag, produced in human cells. Predicted molecular weight: 66.3 kDa. (Accession NP_001125; UniProt P02771) |
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Description : | Alpha-fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistence of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids, and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. The protein belongs to the ALB/AFP/VDB family and contains 3 albumin domains. |
Source : | Human Cells |
Species : | Human |
Tag : | StrepII |
Form : | Lyophilized from sterile 0.2μm filtered solution in 0.3X PBS with 10% Trehalose (carrier-free). |
Protein length : | 20-609, 590 a.a. |
AA Sequence : | TLHRNEYGIASILDSYQCTAEISLA DLATIFFAQFVQEATYKEVSKMVKD ALTAIEKPTGDEQSSGCLENQLPAF LEELCHEKEILEKYGHSDCCSQSEE GRHNCFLAHKKPTPASIPLFQVPEP VTSCEAYEEDRETFMNKFIYEIARR HPFLYAPTILLWAARYDKIIPSCCK AENAVECFQTKAATVTKELRESSLL NQHACAVMKNFGTRTFQAITVTKLS QKFTKVNFTEIQKLVLDVAHVHEHC CRGDVLDCLQDGEKIMSYICSQQDT LSNKITECCKLTTLERGQCIIHAEN DEKPEGLSPNLNRFLGDRDFNQFSS GEKNIFLASFVHEYSRRHPQLAVSV ILRVAKGYQELLEKCFQTENPLECQ DKGEEELQKYIQESQALAKRSCGLF QKLGEYYLQNAFLVAYTKKAPQLTS SELMAITRKMAATAATCCQLSEDKL LACGEGAADIIIGHLCIRHEMTPVN PGVGQCCTSSYANRRPCFSSLVVDE TYVPPAFSDDKFIFHKDLCQAQGVA LQTMKQEFLINLVKQKPQITEEQLE AVIADFSGLLEKCCQGQEQEVCFAE EGQKLISKTRAALGV |
Endotoxin : | <0.1 eu per ug protein by lal |
Purity : | >95% pure by SDS-PAGE |
Storage : | 12 months at -20°C as supplied; 1 month at 4°C after reconstitution. Avoid freeze/thaw cycles. |
Reconstitution : | Centrifuge the vial prior to opening. Reconstitute in PBS to a concentration of 0.1 mg/ml. |
Gene Name : | AFP alpha-fetoprotein [ Homo sapiens ] |
Official Symbol : | AFP |
Synonyms : | AFP; alpha-fetoprotein; HPAFP; FETA; alpha-fetoglobulin; alpha-1-fetoprotein; |
Gene ID : | 174 |
mRNA Refseq : | NM_001134 |
Protein Refseq : | NP_001125 |
UniProt ID : | P02771 |
Chromosome Location : | 4q11-q13 |
Pathway : | Direct p53 effectors, organism-specific biosystem; FOXA2 and FOXA3 transcription factor networks, organism-specific biosystem; Glucocorticoid receptor regulatory network, organism-specific biosystem; |
Function : | metal ion binding; |
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◆ Native Protein | ||
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AFP-3018P | Native pig AFP | +Inquiry |
AFP-412H | Native Human AFP Protein | +Inquiry |
◆ Lysates | ||
AFP-1706HCL | Recombinant Human AFP cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionThe clinical utility of AFP protein as a diagnostic or prognostic marker can be validated through clinical studies using patient samples, correlation analyses with disease outcomes, or comparison with existing diagnostic methods.
Downstream signaling pathways or cellular responses triggered by AFP protein binding or activation can be studied using techniques such as Western blotting, gene expression analysis, or functional assays specific to the signaling pathways of interest.
Therapeutic interventions or treatment strategies targeting AFP protein can be evaluated for efficacy and safety using in vitro or in vivo models, disease-specific assays, or clinical trials to assess their impact on disease progression, patient outcomes, or potential side effects.
The cellular and molecular mechanisms underlying the production and secretion of AFP protein can be elucidated through techniques such as gene expression analysis, protein trafficking studies, or immunofluorescence microscopy.
Genetic variations or mutations in the AFP gene that impact its expression or function can be investigated through genetic association studies, functional characterization of mutant variants, or population-based analyses.
The regulatory factors or signaling pathways that control the expression and transcriptional regulation of the AFP gene can be studied using techniques such as promoter analysis, transcription factor binding assays, or gene knockout/knockdown approaches.
The functional roles of AFP protein in embryonic development, tissue regeneration, or disease progression can be investigated through genetic or pharmacological perturbations, in vitro or in vivo model systems, or functional assays specific to the biological processes of interest.
Specific biomarkers or imaging techniques for detecting AFP protein in biological samples can be developed and their sensitivity and specificity can be assessed using techniques such as ELISA, immunohistochemistry, or imaging modalities like MRI or PET.
The interactions of AFP protein with its binding partners or ligands can be studied using techniques such as co-immunoprecipitation, surface plasmon resonance, or receptor-binding assays.
The immune-related functions of AFP protein, such as immunomodulation or immune evasion, can be investigated using techniques like immune cell assays, cytokine profiling, or immune response assays.
Customer Reviews (3)
Write a reviewInvestigating protein-protein interactions in cellular stress responses for cell survival.
Dissecting protein-protein interactions in viral replication for antiviral strategies.
Mapping protein-protein interactions in protein degradation pathways for proteostasis.
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