Active Recombinant Human INS, His-tagged

• Cat.No.: INS-88H
• Product Overview: Recombinant Human INS(Phe25-Asn110), fused with an N-terminal Met, 6-His tag and Lys, was expressed in E. coli.

Specification

• Description: After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5'' region and with the IGF2 gene at the 3'' region. Alternative splicing results in multiple transcript variants.
• Source: E. coli
• Species: Human
• Tag: His
• Predicted N Terminal: Met
• Form: Lyophilized from a 0.2 μm filtered solution in PBS.
• Bio-activity: Measured in a serum-free cell proliferation assay using MCF 7 human breast cancer cells.
• Molecular Mass: Predicted Molecular Mass: 10.5 kDaSDS-PAGE: 9 kDa, reducing conditions
• Endotoxin: < 1.0 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.

Gene Information

• Gene Name: INS insulin [ Homo sapiens (human) ]
• Official Symbol: INS
• Synonyms: INS; ILPR; IRDN; IDDM1; IDDM2; MODY10; insulin; proinsulin; preproinsulin; insulin-dependent diabetes mellitus 2
• Gene ID: 3630
• mRNA Refseq: NM_000207
• Protein Refseq: NP_000198
• MIM: 176730
• UniProt ID: P01308
• Chromosome Location: 11p15.5
• Pathway: AGE/RAGE pathway; Aldosterone-regulated sodium reabsorption; Cardiac Progenitor Differentiation
• Function: hormone activity; identical protein binding; insulin-like growth factor receptor binding