Recombinant Mouse Gad2 protein(Met1-Leu585), His&GST-tagged

• Cat.No.: Gad2-860M
• Product Overview: Recombinant full length of Mouse GAD2 (NP_032104.2) (Met 1-Leu 585) was expressed in Insect Cells, fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Specification

• Source: Insect Cells
• Species: Mouse
• Tag: N-His&GST
• Protein length: Met1-Leu585
• Form: Lyophilized from sterile 50mM Tris, 100mM NaCl, 0.5mM PMSF, 0.5mM GSH, 3mM DTT, 10% gly, pH 8.0 Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant mouse GAD2/GST chimera consists of 822 amino acids and has a calculated molecular mass of 93 kDa. It migrates as an approximately 80 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Gad2 glutamic acid decarboxylase 2 [ Mus musculus ]
• Official Symbol: Gad2
• Synonyms: GAD2; glutamic acid decarboxylase 2; glutamate decarboxylase 2; GAD-65; 65 kDa glutamic acid decarboxylase; glutamate decarboxylase 65 kDa isoform; GAD65; Gad-2; GAD(65); 6330404F12Rik
• Gene ID: 14417
• mRNA Refseq: NM_008078
• Protein Refseq: NP_032104

Reviews

04/14/2023

The GAD2 protein exhibits remarkable performance in Western blotting (WB) experiments.

12/13/2022

The versatility and efficacy of GAD2 in these applications make it an invaluable tool for researchers.

11/11/2019

Researchers can rely on GAD2 to deliver reliable and accurate results in ELISA experiments, ensuring precise measurement and quantification of target proteins.

Q&As

Is there a genetic component related to GAD2 protein and neurological disorders?

Yes, genetic variations in the GAD2 gene have been associated with an increased risk of developing certain neurological disorders, indicating a genetic component in their etiology.

Can GAD2 protein levels be manipulated pharmacologically?

Pharmacological agents targeting GAD2 activity are being explored to modulate GABAergic neurotransmission, potentially offering novel treatments for neurological disorders.

Can GAD2 protein be used as a diagnostic marker for neurological disorders?

Yes, elevated or reduced levels of GAD2 protein can serve as a diagnostic marker for certain neurological disorders, aiding in their identification and management.

How is GAD2 protein linked to neurological disorders?

GAD2 protein is linked to neurological disorders like epilepsy, Parkinson's disease, and various psychiatric conditions due to its role in regulating GABA levels, which are crucial for proper neuronal function.

Are there any therapeutic interventions targeting GAD2 protein?

Research is ongoing to develop therapeutic interventions that modulate GAD2 activity, aiming to restore GABAergic balance in the brain and potentially treat related neurological disorders.