Native Bovine CAPN2
Cat.No. : | CAPN2-350B |
Product Overview : | CAPN2 was purified by sequential chromatography through DEAE-Sepharose, A 1.5m Bio-Gel, and Phenyl-Sepharose CL-4B columns. |
- Specification
- Gene Information
- Related Products
Source : | Bovine Myocardium. |
Species : | Bovine |
Form : | 50mM Imidazole-HCl, 0.2mM EDTA, 1mM DTT and 50% glycerol, pH 7.4. |
Molecular Mass : | CAPN2 consists of an 80 kDa large subunit and a 30 kDa small subunit. |
Purity : | Greater than 90% as determined by SDS-PAGE. |
Applications : | his protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection. For adsorption we suggest the following procedure: A- Dilute 1μl of the antiserum against m-calpain in 1ml of the usual buffer for immunohistochemistry (final dilution 1:1,000). B- Add 1μg of protein to 1ml of the diluted antibody solution and mix well. C- Incubate for at least 6 hours in the cold. D- Apply to tissue-sections and incubate for 3 days. E- Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB). As a result, the immunostaining should be strongly reduced or even completely prevented. |
Stability : | CAPN2 although stable at 10°C for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
Gene Name : | CAPN2 calpain 2, (m/II) large subunit [ Bos taurus (cattle) ] |
Official Symbol : | CAPN2 |
Synonyms : | calpain-2 catalytic subunit; CANP 2; M-calpaincalpain M-type; millimolar-calpain; calpain-2 large subunit; calpain, large polypeptide L2; calcium-activated neutral proteinase 2 |
Gene ID : | 281662 |
mRNA Refseq : | NM_001103086 |
Protein Refseq : | NP_001096556 |
UniProt ID : | Q27971 |
Chromosome Location : | chromosome: 16 |
Pathway : | Alzheimer"s disease, organism-specific biosystem; Alzheimer"s disease, conserved biosystem; Apoptosis, organism-specific biosystem |
Function : | calcium ion binding; calcium-dependent cysteine-type endopeptidase activity; calcium-dependent cysteine-type endopeptidase activity |
Products Types
◆ Recombinant Protein | ||
CAPN2-0363H | Recombinant Human CAPN2 Protein, GST-Tagged | +Inquiry |
CAPN2-587H | Recombinant Human CAPN2 Protein, His-tagged | +Inquiry |
CAPN2-001P | Recombinant Pig CAPN2 Protein, His-tagged | +Inquiry |
Capn2-362M | Recombinant Mouse Capn2 Protein, GST-tagged | +Inquiry |
CAPN2-496H | Recombinant Human CAPN2 Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Native Protein | ||
CAPN2-22P | Active Native Porcine CAPN2 protein | +Inquiry |
◆ Lysates | ||
CAPN2-7863HCL | Recombinant Human CAPN2 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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