Recombinant Chimpanzee ASAH1 protein, His&Myc-tagged

• Cat.No.: ASAH1-2555C
• Product Overview: Recombinant Chimpanzee ASAH1 protein(A5A6P2)(22-395aa), fused to N-terminal His tag and C-terminal Myc tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Chimpanzee
• Tag: His&Myc
• Form: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
  If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Molecular Mass: 47.7 kDa
• Protein length: 22-395aa
• AA Sequence: QHAPPWTEDCRKSTYPPSGPTYRGP VPWYTINLDLPPYKRWHELMLDKAP MLKVIVNSLKNMINTFVPSGKIVQV VDEKLPGLLGNFPGPFEEEMKGIAA VTDIPLGEIISFNIFYELFTICTSI VAEDKKGHLIHGRNMDFGVFLGWNI NNDTWVITEQLKPLTVNLDFQRNNK TVFKASSFAGYVGMLTGFKPGLFSL SLNERFSINGGYLGILEWILGKKDA MWIGFLTRTVLENSTSYEEAKNLLT KTKILAPAYFILGGNQSGEGCVITR DRKESLDVYELDAKQGRWYVVQTNY DRWKHPFFLDDRRTPAKMCLNRTSQ ENISFETMYDVLSTKPVLNKLTVYT TLIDVTKGQFETYLRDCPDPCIGW
• Purity: Greater than 85% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%.

Reviews

03/21/2022

Such dedicated support makes me feel confident in my choice of using the ASAH1 protein for my experiments.

04/09/2017

The manufacturer's commitment to excellence is truly commendable, especially when it comes to their outstanding technical support.

11/02/2016

The ASAH1 Protein is a protein of exceptional quality that surpasses my expectations and fulfills all my experimental requirements.

Q&As

Can ASAH1 activity be targeted for therapeutic interventions?

Given the involvement of ASAH1 in various diseases, including cancer, modulating its activity or developing ASAH1 inhibitors holds promise as potential therapeutic strategies.

Can alterations in ASAH1 gene expression affect cellular differentiation?

Yes, alterations in ASAH1 gene expression or activity can impact cellular differentiation processes, as ceramide metabolism is crucial for normal development and tissue homeostasis.

Are there any diseases or conditions associated with the ASAH1 protein?

Yes, mutations or deficiencies in the ASAH1 gene are associated with Farber disease, a rare inherited disorder characterized by the accumulation of ceramide in tissues throughout the body.

Can ASAH1 deficiency be prevented?

ASAH1 deficiency, as seen in Farber disease, is a genetic disorder. Prevention involves genetic counseling for at-risk individuals and prenatal testing during pregnancy.

Can ASAH1 deficiency be detected in newborn screening tests?

Currently, ASAH1 deficiency is not included in routine newborn screening panels. However, advancements in testing methods may lead to its inclusion in the future.

Does the ASAH1 protein have any other biological functions?

Besides its role in ceramide metabolism, the ASAH1 protein can affect immune response, inflammation, and cellular stress responses.

How does dysfunction of the ASAH1 protein impact ceramide metabolism?

Dysfunction of the ASAH1 protein impairs the breakdown of ceramide, leading to its accumulation in various tissues and disrupting cellular homeostasis.

Is the ASAH1 protein involved in any signaling pathways?

Yes, the ASAH1 protein can modulate signaling pathways like the sphingosine-1-phosphate pathway, which influences various cellular processes such as migration, inflammation, and angiogenesis.