Recombinant Full Length Human ACAA2 Protein, C-Flag-tagged

• Cat.No.: ACAA2-1365HFL
• Product Overview: Recombinant Full Length Human ACAA2 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: The encoded protein catalyzes the last step of the mitochondrial fatty acid beta-oxidation spiral. Unlike most mitochondrial matrix proteins, it contains a non-cleavable amino-terminal targeting signal.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 41.7 kDa
• AA Sequence: MALLRGVFVVAAKRTPFGAYGGLLK DFTATDLSEFAAKAALSAGKVSPET VDSVIMGNVLQSSSDAIYLA RHVGLRVGIPKETPALTINRLCGSG FQSIVNGCQEICVKEAEVVLCGGTE SMSQAPYCVRNVRFGTKLGS DIKLEDSLWVSLTDQHVQLPMAMTA ENLAVKHKISREECDKYALQSQQRW KAANDAGYFNDEMAPIEVKT KKGKQTMQVDEHARPQTTLEQLQKL PPVFKKDGTVTAGNASGVADGAGAV IIASEDAVKKHNFTPLARIV GYFVSGCDPSIMGIGPVPAISGALK KAGLSLKDMDLVEVNEAFAPQYLAV ERSLDLDISKTNVNGGAIAL
  GHPLGGSGSRITAHLVHELRRRGGK YAVGSACIGGGQGIAVIIQSTATRT RPLEQKLISEEDLAANDILDYKDDD DKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Fatty acid elongation in mitochondria, Fatty acid metabolism, Metabolic pathways, Valine, leucine and isoleucine degradation

Gene Information

• Gene Name: ACAA2 acetyl-CoA acyltransferase 2 [ Homo sapiens (human) ]
• Official Symbol: ACAA2
• Synonyms: DSAEC
• Gene ID: 10449
• mRNA Refseq: NM_006111.3
• Protein Refseq: NP_006102.2
• MIM: 604770
• UniProt ID: P42765

Reviews

02/22/2021

Highly effective in detecting and measuring the activity of protein kinases in kinase assays.

01/21/2021

Provides consistent and accurate quantification of the target protein in different assay formats.

04/05/2017

Shows excellent performance in detecting and measuring the activity of proteases in protease assays.

Q&As

What is the molecular weight of ACAA2 protein?

The molecular weight of ACAA2 protein is approximately 45 kDa.

How does ACAA2 contribute to the pathogenesis of certain types of cancer?

ACAA2 expression is upregulated in some types of cancer, including prostate and ovarian cancer.

How is ACAA2 protein regulated at the transcriptional level?

ACAA2 protein is regulated at the transcriptional level by multiple factors, including PPARs and SREBP-1c.

Where is ACAA2 predominantly expressed in the human body?

ACAA2 is predominantly expressed in the liver and adipose tissue.

What is the clinical significance of ACAA2 protein in human diseases and disorders?

ACAA2 may have clinical significance in the diagnosis and treatment of metabolic disorders and certain types of cancer.

What is the primary function of acetyl-CoA acyltransferase 2 (ACAA2) protein?

ACAA2 protein's primary function is to participate in fatty acid and ketone body metabolism.

What is the role of ACAA2 in the development of metabolic diseases, such as obesity and diabetes?

ACAA2 has been implicated in the development of metabolic disorders, including obesity and type 2 diabetes.

What are the known inhibitors or activators of ACAA2 protein?

Some known inhibitors of ACAA2 include hexachlorophene and fusicoccin-A, while known activators include medium-chain fatty acids and certain drugs.

How does ACAA2 participate in the metabolism of fatty acids and ketone bodies?

ACAA2 catalyzes the transfer of acyl groups between CoA and acyl-CoA substrates.

What is the three-dimensional structure of ACAA2 protein?

The three-dimensional structure of ACAA2 protein is characterized by an alpha/beta hydrolase fold.