Recombinant Full Length Human ACSS3 Protein, C-Flag-tagged

• Cat.No.: ACSS3-481HFL
• Product Overview: Recombinant Full Length Human ACSS3 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Activates acetate so that it can be used for lipid synthesis or for energy generation.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 74.6 kDa
• AA Sequence: MKPSWLQCRKVTSAGGLGGPLPGSS PARGAGAALRALVVPGPRGGLGGRG CRALSSGSGSEYKTHFAASV TDPERFWGKAAEQISWYKPWTKTLE NKHSPSTRWFVEGMLNICYNAVDRH IENGKGDKIAIIYDSPVTNT KATFTYKEVLEQVSKLAGVLVKHGI KKGDTVVIYMPMIPQAMYTMLACAR IGAIHSLIFGGFASKELSSR IDHVKPKVVVTASFGIEPGRRVEYV PLVEEALKIGQHKPDKILIYNRPNM EAVPLAPGRDLDWDEEMAKA QSHDCVPVLSEHPLYILYTSGTTGL PKGVIRPTGGYAVMLHWSMSSIYGL QPGEVWWAASDLGWVVGHSY ICYGPLLHGNTTVLYEGKPVGTPDA GAYFRVLAEHGVAALFTAPTAIRAI RQQDPGAALGKQYSLTRFKT LFVAGERCDVETLEWSKNVFRVPVL DHWWQTETGSPITASCVGLGNSKTP PPGQAGKSVPGYNVMILDDN MQKLKARCLGNIVVKLPLPPGAFSG LWKNQEAFKHLYFEKFPGYYDTMDA GYMDEEGYLYVMSRVDDVIN VAGHRISAGAIEESILSHGTVADCA VVGKEDPLKGHVPLALCVLRKDINA TEEQVLEEIVKHVRQNIGPV
  AAFRNAVFVKQLPKTRSGKIPRSAL SAIVNGKPYKITSTIEDPSIFGHVE EMLKQATRTRPLEQKLISEEDLAAN DILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Pathways: Metabolic pathways, Propanoate metabolism

Gene Information

• Gene Name: ACSS3 acyl-CoA synthetase short chain family member 3 [ Homo sapiens (human) ]
• Official Symbol: ACSS3
• Synonyms: FLJ21963
• Gene ID: 79611
• mRNA Refseq: NM_024560.4
• Protein Refseq: NP_078836.1
• MIM: 614356
• UniProt ID: Q9H6R3

Reviews

11/18/2022

Unbeatable price for the exceptional quality of the protein. Service was impeccable.

02/16/2021

Outstanding quality at an affordable price. Impressed with the value for money.

12/03/2020

Versatile and compatible. Fits various experimental setups."

05/07/2019

"The performance of this protein is truly remarkable. Its consistent and reliable results have significantly contributed to the success of our research endeavors.

08/29/2018

It eliminates background noise and provides clear and interpretable data.

Q&As

What is the significance of ACSS3 in cancer?

ACSS3 has been found to be upregulated in certain cancers and is associated with tumor growth, progression, and metabolic reprogramming.

Can ACSS3 regulate gene expression?

Yes, ACSS3 can influence gene expression through its role in protein acetylation, which can modify histones and transcription factors.

How is ACSS3 activity linked to cellular energy status?

ACSS3 activity is sensitive to cellular energy levels, particularly levels of ATP (adenosine triphosphate), which is an indicator of energy availability.

How is ACSS3 expression regulated?

ACSS3 expression can be regulated by various factors, including nutrient availability, cellular energy status, and transcriptional control.

What are the downstream metabolic pathways that utilize acetyl-CoA?

Acetyl-CoA is involved in various metabolic processes, including the citric acid cycle (Krebs cycle), fatty acid synthesis, and protein acetylation.

Are there any diseases associated with ACSS3?

Recent studies have linked dysregulation of ACSS3 to various diseases, including cancer, obesity, and metabolic disorders.

Are there commercially available antibodies or kits for detecting ACSS3 protein?

Yes, there are commercially available antibodies and kits that can be used for detecting ACSS3 protein in biological samples, such as Western blotting or immunohistochemistry.

Can ACSS3 protein be expressed and purified using recombinant DNA technology?

Yes, ACSS3 protein can be produced using recombinant DNA technology by cloning the ACSS3 gene into an expression vector and expressing it in a suitable host organism, such as bacteria or yeast.

How can the activity of ACSS3 be measured experimentally?

The activity of ACSS3 can be measured through enzymatic assays that assess the conversion of acetate to acetyl-CoA in vitro, using purified ACSS3 protein or cell lysates.

What techniques are commonly used to purify ACSS3 protein for laboratory studies?

Common techniques for purifying ACSS3 protein include affinity chromatography, size exclusion chromatography, and ion exchange chromatography.