Recombinant Full Length Human AFP Protein, C-Flag-tagged

• Cat.No.: AFP-488HFL
• Product Overview: Recombinant Full Length Human AFP Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatocarcinoma and with teratoma, and has prognostic value for managing advanced gastric cancer. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 66.3 kDa
• AA Sequence: MKWVESIFLIFLLNFTESRTLHRNE YGIASILDSYQCTAEISLADLATIF FAQFVQEATYKEVSKMVKDA LTAIEKPTGDEQSSGCLENQLPAFL EELCHEKEILEKYGHSDCCSQSEEG RHNCFLAHKKPTPASIPLFQ VPEPVTSCEAYEEDRETFMNKFIYE IARRHPFLYAPTILLWAARYDKIIP SCCKAENAVECFQTKAATVT KELRESSLLNQHACAVMKNFGTRTF QAITVTKLSQKFTKVNFTEIQKLVL DVAHVHEHCCRGDVLDCLQD GEKIMSYICSQQDTLSNKITECCKL TTLERGQCIIHAENDEKPEGLSPNL NRFLGDRDFNQFSSGEKNIF LASFVHEYSRRHPQLAVSVILRVAK GYQELLEKCFQTENPLECQDKGEEE LQKYIQESQALAKRSCGLFQ KLGEYYLQNAFLVAYTKKAPQLTSS ELMAITRKMAATAATCCQLSEDKLL ACGEGAADIIIGHLCIRHEM TPVNPGVGQCCTSSYANRRPCFSSL VVDETYVPPAFSDDKFIFHKDLCQA QGVALQTMKQEFLINLVKQK
  PQITEEQLEAVIADFSGLLEKCCQG QEQEVCFAEEGQKLISKTRAALGVT RTRPLEQKLISEEDLAANDILDYKD DDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Secreted Protein

Gene Information

• Gene Name: AFP alpha fetoprotein [ Homo sapiens (human) ]
• Official Symbol: AFP
• Synonyms: AFPD; FETA; HPAFP
• Gene ID: 174
• mRNA Refseq: NM_001134.3
• Protein Refseq: NP_001125.1
• MIM: 104150
• UniProt ID: P02771