Recombinant Full Length Human AGPAT2 Protein, GST-tagged

• Cat.No.: AGPAT2-930HF
• Product Overview: Human AGPAT2 full-length ORF ( NP_006403.2, 1 a.a. - 278 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: This gene encodes a member of the 1-acylglycerol-3-phosphate O-acyltransferase family. The protein is located within the endoplasmic reticulum membrane and converts lysophosphatidic acid to phosphatidic acid, the second step in de novo phospholipid biosynthesis. Mutations in this gene have been associated with congenital generalized lipodystrophy (CGL), or Berardinelli-Seip syndrome, a disease characterized by a near absence of adipose tissue and severe insulin resistance. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
• Source: In Vitro Cell Free System
• Species: Human
• Tag: GST
• Molecular Mass: 57.3 kDa
• Protein Length: 278 amino acids
• AA Sequence: MELWPCLAAA LLLLLLLVQL SRAAEFYAKV ALYCALCFTV SAVASLVCLL RHGGRTVENM SIIGWFVRSF KYFYGLRFEV RDPRRLQEAR PCVIVSNHQS ILDMMGLMEV LPERCVQIAK RELLFLGPVG LIMYLGGVFF INRQRSSTAM TVMADLGERM VRENLKVWIY PEGTRNDNGD LLPFKKGAFY LAVQAQVPIV PVVYSSFSSF YNTKKKFFTS GTVTVQVLEA IPTSGLTAAD VPALVDTCHR AMRTTFLHIS KTPQENGATA GSGVQPAQ
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: AGPAT2 1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase, beta) [ Homo sapiens ]
• Official Symbol: AGPAT2
• Synonyms: AGPAT2; 1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase, beta); Berardinelli Seip congenital lipodystrophy, BSCL; 1-acyl-sn-glycerol-3-phosphate acyltransferase beta; LPAAT beta; 1-AGPAT 2; 1-AGP acyltransferase 2; lysophosphatidic acid acyltransferase beta; lysophosphatidic acid acyltransferase-beta; 1-acylglycerol-3-phosphate O-acyltransferase 2 (lysophosphatidic acid acyltransferase-beta); BSCL; BSCL1; LPAAB; 1-AGPAT2; LPAAT-beta
• Gene ID: 10555
• mRNA Refseq: NM_001012727
• Protein Refseq: NP_001012745
• MIM: 603100
• UniProt ID: O15120

Reviews

05/01/2022

 The product’s quality was evident in the absence of background interference in our assays.

10/23/2021

 The product’s cost-effectiveness and clear instructions were valuable in our experiments.

06/18/2021

 The benefits reaped from the product justified its price in terms of scientific progress.

04/09/2020

Consistent outcomes bolstered the reliability of our conclusions, thanks to the product’s performance.

06/26/2016

 Clear, distinct gel bands in electrophoresis highlighted the product’s pure form.

Q&As

How is AGPAT2’s involvement in lipid droplet formation studied

Research investigates AGPAT2’s impact on lipid droplet dynamics and accumulation.

Can AGPAT2’s activity influence brain lipid composition

Yes, AGPAT2’s function can impact brain lipid composition and neurological functions.

How does AGPAT2’s role intersect with phospholipid remodeling

AGPAT2 contributes to phospholipid biosynthesis and remodeling pathways.

How does AGPAT2’s function relate to lipodystrophy

Mutations in AGPAT2 can cause lipodystrophy, leading to abnormal fat tissue distribution.

How does AGPAT2 contribute to phospholipid synthesis

AGPAT2 adds fatty acids to glycerol-3-phosphate, forming lysophosphatidic acid (LPA).

Can AGPAT2’s dysfunction lead to metabolic disorders

Yes, AGPAT2 mutations can result in metabolic disorders and lipodystrophic phenotypes.

Does AGPAT2 contribute to the regulation of inflammation

Yes, AGPAT2’s activity might impact inflammation through lipid metabolism pathways.

How does AGPAT2’s role relate to liver steatosis

AGPAT2’s function can be involved in hepatic lipid accumulation and fatty liver disease.

How is AGPAT2’s function studied in metabolic diseases

Research explores AGPAT2’s role in metabolic disorders like obesity, diabetes, and lipodystrophy.

What’s the role of AGPAT2 in lipid metabolism

AGPAT2 catalyzes the acylation of glycerol-3-phosphate, a crucial step in phospholipid synthesis.

How is AGPAT2’s involvement in triglyceride synthesis studied

Research explores AGPAT2’s contribution to triglyceride synthesis and energy storage.

How does AGPAT2 contribute to lipid composition of tissues

AGPAT2’s activity influences the lipid composition of various tissues.

How is AGPAT2’s connection to lipogenesis studied

Research explores AGPAT2’s role in de novo lipogenesis and fatty acid synthesis.

Is AGPAT2 essential for normal cellular function

Yes, AGPAT2 is crucial for lipid metabolism and the synthesis of cellular membranes.

Can AGPAT2 be targeted for therapeutic interventions

Yes, AGPAT2 is a potential target for modulating lipid metabolism and related diseases.

Can AGPAT2 play a role in membrane fusion processes

Yes, AGPAT2’s involvement in lipid synthesis can influence membrane fusion and trafficking.

Where is AGPAT2 located in cells

AGPAT2 is primarily located in the endoplasmic reticulum (ER) membrane.

How is AGPAT2’s involvement in glycerophospholipid metabolism studied

Research investigates AGPAT2’s role in lipid biosynthesis pathways.

Can AGPAT2’s activity impact cellular membranes

Yes, AGPAT2’s function is essential for maintaining the composition and integrity of cellular membranes.

Can AGPAT2 influence adipocyte differentiation

Yes, AGPAT2’s activity is linked to adipocyte differentiation and fat tissue development.

How is AGPAT2’s structure characterized

AGPAT2 contains transmembrane domains that allow it to function within membranes.